| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,434,377 | A→G | F222S (TTC→TCC) | nifJ ← | pyruvate:ferredoxin (flavodoxin) oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,434,377 | 0 | A | G | 95.9% | 148.6 / ‑0.9 | 49 | F222S (TTC→TCC) | nifJ | pyruvate:ferredoxin (flavodoxin) oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (31/16); total (32/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01 | |||||||||||
TGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGAC > NZ_CP009273/1434292‑1434462 | tGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATa > 2:795757/1‑90 (MQ=255)tGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATa > 1:141859/1‑90 (MQ=255) ggCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTc < 2:574392/90‑1 (MQ=255) ggCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTc < 1:1074563/90‑1 (MQ=255) tcatCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACg > 2:1299814/1‑90 (MQ=255) atCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTa > 2:1480008/1‑90 (MQ=255) gCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACcg > 1:214871/1‑90 (MQ=255) tGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCgg < 2:974827/90‑1 (MQ=255) gTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAg > 1:1289904/1‑63 (MQ=255) gTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAg < 2:1289904/63‑1 (MQ=255) gTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGa > 2:1058364/1‑90 (MQ=255) gTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGa > 2:335326/1‑90 (MQ=255) gTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGa > 2:159446/1‑90 (MQ=255) gTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGa > 1:249109/1‑90 (MQ=255) tGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGa < 2:583461/90‑1 (MQ=255) gTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAAt > 2:1161487/1‑50 (MQ=255) gTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAAt < 1:1161487/50‑1 (MQ=255) gTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATg > 2:281940/1‑90 (MQ=255) gTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATg > 2:1051431/1‑90 (MQ=255) ccGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGt < 1:349700/90‑1 (MQ=255) gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTAGCGGGGATCACCGGGTGTTCCGGGTTg > 1:1473726/1‑90 (MQ=255) gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCCCCGGATGTTCCGGGGTg > 1:931252/1‑90 (MQ=255) gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGGTGGTCCGGGTTg > 1:1412309/1‑90 (MQ=255) gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg > 1:170714/1‑90 (MQ=255) gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg > 1:1411241/1‑90 (MQ=255) gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg > 2:372240/1‑90 (MQ=255) gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg > 1:767317/1‑90 (MQ=255) gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg > 1:407944/1‑90 (MQ=255) gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTg < 1:297320/90‑1 (MQ=255) gcgTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGGTCCGGGTTg > 1:1316310/1‑90 (MQ=255) aCCATGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGccc < 2:1411241/90‑1 (MQ=255) aTGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCggg > 2:872787/1‑90 (MQ=255) aTGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCggg > 1:154421/1‑90 (MQ=255) aTGGGTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCggg > 2:1486087/1‑90 (MQ=255) gggTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGgcg < 1:1397349/90‑1 (MQ=255) ggTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGgcgc > 2:146434/1‑90 (MQ=255) ggTTGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGgcgc < 1:335326/90‑1 (MQ=255) ttGGTGGCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGa > 2:317309/1‑90 (MQ=255) gtggCTTCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGa < 1:1120623/90‑1 (MQ=255) ttCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATc < 2:214871/90‑1 (MQ=255) ttCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATc > 2:1118673/1‑90 (MQ=255) ttCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATc < 1:1233058/90‑1 (MQ=255) ttCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATc > 2:78216/1‑90 (MQ=255) ttCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATc < 1:566714/90‑1 (MQ=255) tCGCGAGACTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCa > 2:465539/1‑90 (MQ=255) tCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCgg < 1:1397562/45‑1 (MQ=255) tCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCgg > 2:1397562/1‑45 (MQ=255) gaCTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCg < 2:636947/90‑1 (MQ=255) cTGGGAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGAc > 2:1108555/1‑90 (MQ=255) | TGTCGCGGCAGAGAAATCATTCATCGCCTGTTCAACATGGTCATAGACCGCGTTGTACCATGGGTTGGTGGCTTCGCGAGACTGGAAATAAGTGTCAGGATTGGCGGACGTACCGCGGATCACCGGATGTTCCGGGTTGAGTGCCCGGGCGCGATGAGCATCAATTTCGAC > NZ_CP009273/1434292‑1434462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |