| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,454,774 | A→G | D16G (GAT→GGT) | paaI → | hydroxyphenylacetyl‑CoA thioesterase PaaI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,454,774 | 0 | A | G | 98.1% | 169.3 / ‑3.7 | 53 | D16G (GAT→GGT) | paaI | hydroxyphenylacetyl‑CoA thioesterase PaaI |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (18/34); total (18/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.32e-01 | |||||||||||
GTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAA > NZ_CP009273/1454689‑1454857 | gTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGcc < 1:449282/90‑1 (MQ=255) ttGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGAATGGGAACGGGGCCTgcgc > 2:1315714/1‑90 (MQ=255) gcgcCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAg > 2:37714/1‑90 (MQ=255) gcgcCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAg > 1:1171616/1‑90 (MQ=255) gcgcCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAg > 1:1499258/1‑90 (MQ=255) gcgcTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCAt < 2:363313/90‑1 (MQ=255) gcTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCg < 2:510293/90‑1 (MQ=255) cTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACAt < 2:126803/90‑1 (MQ=255) tGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACAtt < 1:1128129/90‑1 (MQ=255) gCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCa > 1:1289177/1‑90 (MQ=255) gCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCa > 2:244003/1‑90 (MQ=255) gCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCa > 2:321234/1‑90 (MQ=255) gCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCa > 1:185576/1‑90 (MQ=255) ggTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAAt < 2:394777/90‑1 (MQ=255) tATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCg > 2:385235/1‑74 (MQ=255) tATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCg < 1:385235/74‑1 (MQ=255) aGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGaa < 1:1074347/90‑1 (MQ=255) cATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc < 2:426682/90‑1 (MQ=255) cATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc < 2:655354/90‑1 (MQ=255) cATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc < 1:1002127/90‑1 (MQ=255) cATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc < 2:948552/90‑1 (MQ=255) cATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc < 2:1210453/90‑1 (MQ=255) cATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc < 1:686778/90‑1 (MQ=255) aTAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCt > 1:1503172/1‑90 (MQ=255) aTAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCt > 2:889206/1‑90 (MQ=255) aTAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCt > 1:1312397/1‑90 (MQ=255) tAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCtt < 1:903242/90‑1 (MQ=255) tAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCtt < 1:612168/90‑1 (MQ=255) tAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCtt < 2:757857/90‑1 (MQ=255) ggCCTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGc < 1:301082/90‑1 (MQ=255) cTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGt > 1:704300/1‑90 (MQ=255) cTGGCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGt > 2:651703/1‑90 (MQ=255) ggCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAg < 1:1288494/90‑1 (MQ=255) ggCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAg > 2:1471460/1‑90 (MQ=255) ggCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAg < 2:159197/90‑1 (MQ=255) ggCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAg < 2:79742/90‑1 (MQ=255) ggCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAg < 1:244003/90‑1 (MQ=255) gCAAAATGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGt < 2:108308/90‑1 (MQ=255) aaTGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGAcc > 1:1096202/1‑90 (MQ=255) aTGCCCATGCTATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCa < 2:658118/90‑1 (MQ=255) aTGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCa < 2:1579763/90‑1 (MQ=255) aTGCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCa < 1:766273/90‑1 (MQ=255) gCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATg < 2:439319/90‑1 (MQ=255) gCCCATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATg < 2:1312397/90‑1 (MQ=255) cATGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGAcc < 1:975073/90‑1 (MQ=255) aTGCAATGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCg > 2:538181/1‑90 (MQ=255) aTGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACt < 2:498522/90‑1 (MQ=255) aTGTATGAGAACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACt < 1:68520/90‑1 (MQ=255) agaACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACaaa < 1:497041/90‑1 (MQ=255) agaACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACaaa < 1:567255/90‑1 (MQ=255) gaACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAAt > 2:770731/1‑45 (MQ=255) gaACGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAAt < 1:770731/45‑1 (MQ=255) aCGGTGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTc < 2:1133814/77‑1 (MQ=255) | GTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAA > NZ_CP009273/1454689‑1454857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |