| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,505,430 | A→G | K391E (AAA→GAA) | ydcR → | PLP‑dependent aminotransferase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,505,430 | 0 | A | G | 94.9% | 108.4 / NA | 39 | K391E (AAA→GAA) | ydcR | PLP‑dependent aminotransferase family protein |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (23/14); total (23/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGAC > NZ_CP009273/1505344‑1505518 | cccATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGgaaa < 2:486986/90‑1 (MQ=255) ccATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAAt > 2:1211102/1‑90 (MQ=255) gtcgCCTGCGTCGCCAGCTCGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATa < 2:1341041/90‑1 (MQ=255) gCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATg > 1:971666/1‑90 (MQ=255) gCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATg > 2:41216/1‑90 (MQ=255) gCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATg > 1:594152/1‑90 (MQ=255) gCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAg > 1:407194/1‑90 (MQ=255) gCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAg > 2:214356/1‑90 (MQ=255) tGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTtct > 1:1567164/1‑90 (MQ=255) acGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTccc > 1:211373/1‑90 (MQ=255) cGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTcccc > 1:16976/1‑90 (MQ=255) gTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCg > 1:1470705/1‑90 (MQ=255) tGCCTGGCAGCCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGTTTACTTTCTCTGGTTGGAGCCCCCCGa < 1:833095/90‑1 (MQ=255) tGCCGGGTAGGCCCGGTTGGGTTATCTGCCGGGGGAAGCGGAAATTCATTAAAAGGACAGTGGTTACTTTCCCTGGTTGGAGCCCCCCGa < 1:982051/90‑1 (MQ=255) gCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAg < 2:105694/90‑1 (MQ=255) tGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACa > 1:646361/1‑55 (MQ=255) tGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACa < 2:646361/55‑1 (MQ=255) ggcaggcaCTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGt > 2:407682/1‑90 (MQ=255) caggcaCTGCTGCTTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTa < 1:653250/90‑1 (MQ=255) ggcaCTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGa > 2:592925/1‑90 (MQ=255) gctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCgg > 1:320868/1‑90 (MQ=255) ctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGc < 1:41216/90‑1 (MQ=255) ctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGc < 2:16976/90‑1 (MQ=255) ctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGc < 2:1329800/90‑1 (MQ=255) ctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGc < 2:606747/90‑1 (MQ=255) ctgcGTTATCTGCCTGCGGAAGGGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGc < 1:1018435/90‑1 (MQ=255) cGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGaa > 1:945007/1‑90 (MQ=255) tATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGTTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTa < 2:686833/90‑1 (MQ=255) aTCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTaa > 1:1404976/1‑90 (MQ=255) ctgcctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGc > 2:842747/1‑90 (MQ=255) cctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTg > 1:943248/1‑90 (MQ=255) cctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTg > 2:442352/1‑90 (MQ=255) cctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTg > 1:1308888/1‑90 (MQ=255) tgcGGAAGGGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTggc < 2:898376/90‑1 (MQ=255) gcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAg < 2:740496/60‑1 (MQ=255) gcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAg > 1:740496/1‑60 (MQ=255) gcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTggcg > 2:1471245/1‑90 (MQ=255) gcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTggcg > 1:1224969/1‑90 (MQ=255) ggAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTggcggc < 2:1224969/90‑1 (MQ=255) gAAAATTCATCATAATGACAGTGGTTCTTTTCTCTGGTTGGAGCCCCCGGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGAc < 2:896939/90‑1 (MQ=255) | CCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGAC > NZ_CP009273/1505344‑1505518 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |