| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 115,628 | A→C | T137P (ACG→CCG) | ampD → | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 115,628 | 0 | A | C | 98.4% | 173.5 / NA | 65 | T137P (ACG→CCG) | ampD | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Reads supporting (aligned to +/- strand): ref base A (0/1); major base C (35/28); minor base T (1/0); total (36/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGAT > NZ_CP009273/115542‑115711 | gCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCcgc < 1:556738/90‑1 (MQ=255)gCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCcgc > 2:489286/1‑90 (MQ=255) aaTGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCgg < 2:504481/90‑1 (MQ=255) aTTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCAc < 1:292941/90‑1 (MQ=255) ttttCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACtg > 1:1464886/1‑90 (MQ=255) ttCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGGTCCGCGGGGACtgat > 2:1107636/1‑90 (MQ=255) aTTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACtgattgat < 2:393673/90‑1 (MQ=255) ggATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCt < 2:944546/90‑1 (MQ=255) gATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTa < 1:1142299/90‑1 (MQ=255) ttGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATc < 1:544187/90‑1 (MQ=255) gCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTa < 2:929687/90‑1 (MQ=255) cACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTGATTGATTGCTATCCGGATATCGCTaa > 2:149993/1‑90 (MQ=255) cACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTGATTGATTGCGATCCGGATATCGCTaa > 1:798536/1‑90 (MQ=255) aCCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTGGTTTATTTCTATCCGGGTAACGCTaaa > 1:763271/1‑90 (MQ=255) gATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAAc < 1:68679/90‑1 (MQ=255) cGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGa < 1:704155/90‑1 (MQ=255) cTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACg > 2:541661/1‑90 (MQ=255) cTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACg < 2:579188/90‑1 (MQ=255) cTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACg < 1:1137914/90‑1 (MQ=255) cTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACg > 2:1478962/1‑90 (MQ=255) ggCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACggg > 2:604343/1‑90 (MQ=255) ggCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACggg > 2:304653/1‑90 (MQ=255) cGTATTCCGATGCGTCGGATCAACATCTTGCGGCGGTTCCGCGGGCTCTGATTGATTGGTATCCGGATATCGCTAAAAGCATGACGGGcc < 1:1260717/90‑1 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 1:1211628/90‑1 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 1:1489022/90‑1 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 2:1320343/90‑1 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 1:785556/90‑1 (MQ=255) gTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTGATTGATTTCTATCCGGATATTGCTAAAAAAATGACGGGGCa > 1:88390/1‑90 (MQ=255) tataCCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCAt < 2:600446/90‑1 (MQ=255) tataCCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCAt < 1:922760/90‑1 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTTCGCGGGc > 2:1193389/1‑38 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGc < 1:1193389/38‑1 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGTTTTCTATTCGGGTATCGCTAAAAACATGACGGGGCCTTTTGat > 1:879358/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTTTCCGGATATCGCTAAAAACATGACGGGGCACTGTGat > 2:1236679/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGgtat > 2:478818/1‑62 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGatat < 1:478818/62‑1 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGa < 1:1181395/76‑1 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGa > 2:1181395/1‑76 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGat > 2:498911/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAAAATGACGGGCCATTGTGat > 1:893490/1‑90 (MQ=255) aTGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGata > 2:758611/1‑90 (MQ=255) gcgcAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATAtt > 1:483911/1‑90 (MQ=255) gcAGTATCAACAGCTTGCGGCGGTTCCGTGGGCACTGGTTGATTGCTATCCGGATATCGCTAAAAAAATGACGGGCCCTTGTGATATTgc > 1:83911/1‑90 (MQ=255) gcAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAAAATGACGGGCCATTGTGATATTgc > 2:478345/1‑90 (MQ=255) cAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTGATTGGTTTCTATCCGGATATCGCTAAAAACATGACGGGCCATTTTGATATTgcg > 2:1257475/1‑90 (MQ=255) aTCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCgg < 2:5233/90‑1 (MQ=255) aGCTTGCGGCGGTTCCGCGGGCCCTTCTTTATTGCTATCCGGATATCGCTAAAAAAATGACGGGCCATTTTGATATTGCGCCGGGTCGGa > 2:1109994/1‑90 (MQ=255) aGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAAAATGACGGGCCATTGTGATATTTCGCCGGATCGGa > 1:299360/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGCTTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGaaa < 1:1368657/90‑1 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTTCTATCCGGGTATCGCTAAAAAAATGACGGGGCATTTTGGTATTTCGCCGGGTCGGgaa > 1:871034/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATtgt > 1:1373755/1‑69 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATtgt < 2:1373755/69‑1 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGGTATTTCGCCGGATCGGaaa > 1:570750/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGaaa > 1:440749/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGaaa > 2:1368657/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGaaa > 2:618926/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAAAATGACGGGCCATTGTGATATTGCGCCGGATCGGaaa > 1:605270/1‑90 (MQ=255) ttGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAAc > 1:1254043/1‑53 (MQ=255) ttGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAAc < 2:1254043/53‑1 (MQ=255) tGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGatat > 2:479125/1‑42 (MQ=255) tGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGatat < 1:479125/42‑1 (MQ=255) tGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAAc > 2:533783/1‑90 (MQ=255) tGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAAc < 1:1086624/90‑1 (MQ=255) cggcggTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCg < 2:1226707/90‑1 (MQ=255) gcggTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGAt > 2:516890/1‑90 (MQ=255) | GCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGAT > NZ_CP009273/115542‑115711 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |