Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,575,825	A→T	D319E (GAT→GAA)	ydeN ←	sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,575,825	0	A	T	98.5%	259.6 / ‑4.8	68	D319E (GAT→GAA)	ydeN	sulfatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (31/36);  minor base C (1/0);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

CACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGATCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATCCGGTGCAGGATT  >  NZ_CP009273/1575740‑1575911                                                                                      |                                                                                       cACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCaa                                                                                    >  2:673867/1‑90 (MQ=255)  aCCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAAc                                                                                   <  2:361386/90‑1 (MQ=255)  aCCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAAc                                                                                   <  2:213150/90‑1 (MQ=255)  aCCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAAc                                                                                   <  1:530872/90‑1 (MQ=255)   ccATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACa                                                                                  <  2:1065743/90‑1 (MQ=255)             aGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAAc                                                                                   >  2:1440466/1‑79 (MQ=255)             aGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAAc                                                                                   <  1:1440466/79‑1 (MQ=255)             aGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACgg                                                                        >  1:108857/1‑90 (MQ=255)             aGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACgg                                                                        >  2:1140263/1‑90 (MQ=255)             aGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACgg                                                                        >  1:451528/1‑90 (MQ=255)              ggTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGa                                                                       <  1:1475473/90‑1 (MQ=255)                tAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAg                                                                     >  1:1478330/1‑90 (MQ=255)                   agagAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCgt                                                                  <  2:462552/90‑1 (MQ=255)                   agagAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCgt                                                                  <  1:673867/90‑1 (MQ=255)                      gaATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCgtagt                                                               <  2:673460/90‑1 (MQ=255)                      gaATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCgtagt                                                               >  2:603981/1‑90 (MQ=255)                        ataatTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGtagtag                                                             >  2:188682/1‑90 (MQ=255)                              gtattgtCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATct                                                       >  1:525162/1‑90 (MQ=255)                               tattgtCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATctg                                                      <  1:1301039/90‑1 (MQ=255)                                attgtCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATctgc                                                     >  2:349066/1‑90 (MQ=255)                                attgtCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATctgc                                                     >  2:507232/1‑90 (MQ=255)                                attgtCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATctgc                                                     >  2:59358/1‑90 (MQ=255)                                   gtCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGt                                                  >  2:764301/1‑90 (MQ=255)                                   gtCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGt                                                  >  1:878680/1‑90 (MQ=255)                                        aCTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGAc                                             >  1:129607/1‑90 (MQ=255)                                        aCTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGAc                                             >  1:582923/1‑90 (MQ=255)                                          tGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTa                                           >  2:125930/1‑90 (MQ=255)                                           gTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAc                                          >  1:147350/1‑90 (MQ=255)                                           gTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAc                                          >  2:1393493/1‑90 (MQ=255)                                           gTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAc                                          >  1:1409882/1‑90 (MQ=255)                                           gTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAc                                          >  1:329892/1‑90 (MQ=255)                                           gTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAc                                          >  1:1414283/1‑90 (MQ=255)                                           gTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGCTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAc                                          >  2:250160/1‑90 (MQ=255)                                            tCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAcc                                         <  1:809932/90‑1 (MQ=255)                                            tCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAcc                                         <  1:1382961/90‑1 (MQ=255)                                            tCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAcc                                         <  2:888936/90‑1 (MQ=255)                                            tCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAcc                                         <  1:1414916/90‑1 (MQ=255)                                            tCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAcc                                         <  2:1414283/90‑1 (MQ=255)                                            tCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAcc                                         <  2:1281731/90‑1 (MQ=255)                                            tCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAcc                                         <  2:1330551/90‑1 (MQ=255)                                             ccGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAcc                                         <  2:353864/89‑1 (MQ=255)                                             ccGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTAcc                                         >  1:353864/1‑89 (MQ=255)                                             ccGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCg                                        >  1:1184263/1‑90 (MQ=255)                                             ccGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCg                                        >  1:414264/1‑90 (MQ=255)                                             ccGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCg                                        >  1:767384/1‑90 (MQ=255)                                             ccGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCg                                        >  2:548335/1‑90 (MQ=255)                                                      ttcAGTTGTTCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAAt                               <  2:491649/90‑1 (MQ=255)                                                            tgttCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTc                         <  1:1456441/90‑1 (MQ=255)                                                             gttCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCt                        <  2:767384/90‑1 (MQ=255)                                                             gtccGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCt                        <  1:968911/87‑1 (MQ=255)                                                              ttCGAGAATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTg                       <  2:245071/90‑1 (MQ=255)                                                                   gaATGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATAtt                  >  1:1027825/1‑90 (MQ=255)                                                                    aaTGCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTg                 >  2:565509/1‑90 (MQ=255)                                                                       gCGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATc              >  1:9187/1‑90 (MQ=255)                                                                        cGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATcc             <  2:414264/90‑1 (MQ=255)                                                                        cGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATcc             <  2:505135/90‑1 (MQ=255)                                                                        cGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATcc             <  2:1409882/90‑1 (MQ=255)                                                                        cGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATcc             <  1:764301/90‑1 (MQ=255)                                                                        cGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATcc             <  1:548335/90‑1 (MQ=255)                                                                        cGTTTTACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATcc             <  1:1237182/90‑1 (MQ=255)                                                                            ttACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATCCGGTg         <  1:1184717/90‑1 (MQ=255)                                                                             tACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATCCGGTGc        <  1:507232/90‑1 (MQ=255)                                                                             tACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATCCGGTGc        <  1:181629/90‑1 (MQ=255)                                                                             tACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATCCGGTGc        <  1:1291666/90‑1 (MQ=255)                                                                             tACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATCCGGTGc        <  1:125930/90‑1 (MQ=255)                                                                             tACACCCTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATCCGGTGc        <  2:933114/90‑1 (MQ=255)                                                                                   cTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATCCGGTGCAGGAtt  >  1:705686/1‑90 (MQ=255)                                                                                   cTGTTCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATCCGGTGCAGGAtt  <  2:697198/90‑1 (MQ=255)                                                                                      |                                                                                       CACCATTATCGGAGGTAAAGAGAATAATTGTATTGTCATACTGTCCGTTTTTCTTCAGTTGTTCGAGAATGCGTTTTACACCCTGATCAACAGAATAAACGGAAGCGTAGTAGTTATCTGCTGTTTGACTACCGGTATTAAATTGCTTCTGATATTGATCCGGTGCAGGATT  >  NZ_CP009273/1575740‑1575911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑