| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,579,109 | A→C | G545G (GGT→GGG) | ydeP ← | acid resistance putative oxidoreductase YdeP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,579,109 | 0 | A | C | 95.8% | 229.8 / 1.6 | 72 | G545G (GGT→GGG) | ydeP | acid resistance putative oxidoreductase YdeP |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base C (37/32); total (40/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-01 | |||||||||||
CAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACACCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTTTGC > NZ_CP009273/1579025‑1579193 | caGATACTCCCAGTCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggc < 1:1372443/90‑1 (MQ=255)caGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggc < 1:360032/90‑1 (MQ=255)caGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggc < 1:103644/90‑1 (MQ=255)caGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggc < 2:1196997/90‑1 (MQ=255) aGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggcg < 1:669846/90‑1 (MQ=255) gATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggcgg < 2:620199/90‑1 (MQ=255) cccAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTa > 2:430215/1‑90 (MQ=255) ggCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAacac > 2:233753/1‑90 (MQ=255) cTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAAcacgc < 1:1493923/90‑1 (MQ=255) cTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAAcacgc > 1:1498984/1‑90 (MQ=255) aCCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAAcacgcca > 2:133667/1‑90 (MQ=255) aCCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAAcacgcca > 2:170525/1‑90 (MQ=255) aCCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAAcacgcca > 2:467316/1‑90 (MQ=255) cGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCAcgcg < 1:1553280/90‑1 (MQ=255) gCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTCCCCCGGCGGGTTTTAACACGCCACGCGa < 1:1348218/90‑1 (MQ=255) ctctGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAg > 2:135820/1‑90 (MQ=255) tGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCa < 1:1418816/90‑1 (MQ=255) gTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggc < 1:732955/58‑1 (MQ=255) gTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggc > 2:732955/1‑58 (MQ=255) gTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatca > 1:1042816/1‑90 (MQ=255) gTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatca > 2:473355/1‑90 (MQ=255) gTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatca > 1:1473113/1‑90 (MQ=255) gTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatca > 1:429662/1‑90 (MQ=255) gTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatca > 1:1269643/1‑90 (MQ=255) gTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACACCGGCGGGGTTTAACAACCCCCCCGGGGGATGAaaca > 1:1120714/1‑90 (MQ=255) gctgcCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatcatc > 2:578910/1‑90 (MQ=255) gctgcCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatcatc > 2:1252709/1‑90 (MQ=255) gctgcCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatcatc < 2:1269643/90‑1 (MQ=255) gctgcCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatcatc > 1:1280370/1‑90 (MQ=255) gctgcCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatcatc > 2:1487295/1‑90 (MQ=255) tgcctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGa < 2:1498984/90‑1 (MQ=255) tgcctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGa < 2:269089/90‑1 (MQ=255) tgcctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGa < 2:678296/90‑1 (MQ=255) gcctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggc < 1:652418/53‑1 (MQ=255) gcctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggc > 2:652418/1‑53 (MQ=255) gcctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGAc > 2:100849/1‑90 (MQ=255) ctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACAt > 1:191216/1‑90 (MQ=255) gcgcGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACAtt > 2:450439/1‑89 (MQ=255) gcgcGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACAtt < 1:450439/89‑1 (MQ=255) gcgcGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTg > 1:1382465/1‑90 (MQ=255) gcgcGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTg > 2:647340/1‑90 (MQ=255) gcgcGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTg < 1:198300/90‑1 (MQ=255) gcgcGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTg > 2:366627/1‑90 (MQ=255) gcgcGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTg < 2:1473113/90‑1 (MQ=255) gcgcGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTg > 1:424022/1‑90 (MQ=255) cgcgATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTg < 2:701387/89‑1 (MQ=255) cgcgATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTg > 1:701387/1‑89 (MQ=255) gcgATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGaa < 2:991963/90‑1 (MQ=255) gcgATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGaa < 2:1382465/90‑1 (MQ=255) gATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATc > 1:665549/1‑90 (MQ=255) ttCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCt > 1:440374/1‑90 (MQ=255) cgcgACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAAc < 2:1550133/90‑1 (MQ=255) cgcgACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAAc > 2:1075066/1‑90 (MQ=255) cgcgACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAAc < 1:1552941/90‑1 (MQ=255) cgcgACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAAc < 2:429662/90‑1 (MQ=255) gcgACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACg > 1:454862/1‑90 (MQ=255) gcgACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACg > 2:312656/1‑90 (MQ=255) gACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGt > 1:656894/1‑90 (MQ=255) gACCACTGCACACTCTGATTTCAGCATTACACCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGt > 2:1007761/1‑90 (MQ=255) ccACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTa > 1:299602/1‑90 (MQ=255) aCTGCACACTCTGTTTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTAcc < 2:383575/90‑1 (MQ=255) aCTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTAcc < 1:366627/90‑1 (MQ=255) gCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACCCGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGGTACCGcc > 2:1499886/1‑90 (MQ=255) ctctGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATTAATCATCGACATTGAATCCTCAACGGTTACCGCCTgcgc < 2:1422445/90‑1 (MQ=255) ctctGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTgcgc > 2:834410/1‑90 (MQ=255) ctGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAGTCCTCAACGGTTACCGCCTGCGCAc < 1:402409/90‑1 (MQ=255) ctGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCAc < 1:170525/90‑1 (MQ=255) ctGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCAc < 2:771037/90‑1 (MQ=255) ctGATTTCAGCATTACACCGGCGGGGTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCCGCGCAc > 1:77363/1‑90 (MQ=255) tGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCAcc > 2:410737/1‑90 (MQ=255) aGCATTACCCCGGCGGGATTTGATCCGCCACGCGAGGCTTGAATCATGGAGATTGAATCCTCAACGGGTACCTCCTACGCACCGgttttt > 1:857951/1‑90 (MQ=255) aTTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTTTGc < 1:410737/90‑1 (MQ=255) | CAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACACCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTTTGC > NZ_CP009273/1579025‑1579193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |