Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	139,618	A→G	E118G (GAA→GGA)	yadG →	ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	139,618	0	A	G	100.0%	240.9 / NA	73	E118G (GAA→GGA)	yadG	ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/34);  total (39/34)

AACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCG  >  NZ_CP009273/139533‑139696                                                                                      |                                                                               aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                                            <  1:471564/90‑1 (MQ=255) aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                                            <  2:769163/90‑1 (MQ=255) aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                                            <  1:679768/90‑1 (MQ=255) aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                                            <  1:26291/90‑1 (MQ=255) aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                                            <  2:17785/90‑1 (MQ=255) aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGAGTACATCCGCAGCGGAAAg                                                                            <  1:1532280/90‑1 (MQ=255)     cGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATc                                                                        <  2:655984/90‑1 (MQ=255)          gAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTaaa                                                                   <  2:230463/90‑1 (MQ=255)                gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTAGATCCGCAGCGGAAAGTATCCTAAACAACTc                                                             >  1:820473/1‑90 (MQ=255)                gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACt                                                              <  2:1484511/89‑1 (MQ=255)                gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACt                                                              >  1:1484511/1‑89 (MQ=255)                gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc                                                             <  2:1380207/90‑1 (MQ=255)                gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc                                                             <  2:746192/90‑1 (MQ=255)                    agcaAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATc                                                         <  2:283227/90‑1 (MQ=255)                     gcaAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCt                                                        >  1:1564501/1‑90 (MQ=255)                          ttGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATggg                                                   >  1:330466/1‑90 (MQ=255)                              ggtgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGaaa                                               <  2:1564501/90‑1 (MQ=255)                              ggtgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGaaa                                               <  1:564556/90‑1 (MQ=255)                               gtgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGaaaa                                              >  2:1206547/1‑90 (MQ=255)                                   aTCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCa                                          >  1:1036595/1‑90 (MQ=255)                                   aTCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCa                                          >  1:818749/1‑90 (MQ=255)                                   aTCAGGCAGGGTACTACGGCGTGGAGCGCAAAAAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCa                                          >  1:917824/1‑90 (MQ=255)                                      aggcaggGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCaacg                                       >  1:1283505/1‑90 (MQ=255)                                      aggcaggGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGGATCTTAAACAACTCGGTCTATGGGGAAAACGCaacg                                       >  1:925092/1‑90 (MQ=255)                                          aggGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCaacgaacg                                   <  2:854969/90‑1 (MQ=255)                                           gggTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGATCGt                                  >  2:266151/1‑90 (MQ=255)                                           gggTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGt                                  >  2:514307/1‑90 (MQ=255)                                           gggTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGt                                  >  2:223806/1‑90 (MQ=255)                                           gggTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGt                                  >  2:1255550/1‑90 (MQ=255)                                             gTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTgc                                >  1:589734/1‑90 (MQ=255)                                             gTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTgc                                <  1:882336/90‑1 (MQ=255)                                              tactacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTgcg                               <  2:997242/90‑1 (MQ=255)                                               actacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTgcgc                              <  2:1402250/90‑1 (MQ=255)                                               actacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTgcgc                              <  1:1060722/90‑1 (MQ=255)                                                ctacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGcgcg                             <  1:1306427/90‑1 (MQ=255)                                                  acGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTa                           >  1:1283333/1‑90 (MQ=255)                                                  acGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTa                           <  1:1453366/90‑1 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                                            <  1:1264190/38‑1 (MQ=38)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                                            >  2:1264190/1‑38 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGaa                                                >  2:853249/1‑66 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCaacgaacg                                   <  1:1555581/79‑1 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCaacgaacg                                   >  2:1555581/1‑79 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt                        >  1:489443/1‑90 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt                        >  1:190084/1‑90 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt                        >  2:1046988/1‑90 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt                        >  1:603724/1‑90 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt                        >  1:875248/1‑90 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGGGCGCGTATGt                        >  2:322425/1‑90 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTACCTTAAACAACTCGATCTATGGGGaa                                                <  1:853249/66‑1 (MQ=255)                                                       gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                                            >  2:364148/1‑36 (MQ=255)                                                       gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                                            <  1:364148/36‑1 (MQ=38)                                                       gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                      >  2:1095001/1‑90 (MQ=255)                                                       gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                      >  2:194827/1‑90 (MQ=255)                                                       gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                      >  1:975551/1‑90 (MQ=255)                                                       gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                      >  1:970862/1‑90 (MQ=255)                                                       gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa                      >  1:1007495/1‑90 (MQ=255)                                                            gcgcAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTgg                 >  2:1251475/1‑90 (MQ=255)                                                              gcAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCg               >  1:1242142/1‑90 (MQ=255)                                                              gcAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCg               >  1:345381/1‑90 (MQ=255)                                                              gcAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCg               <  2:330466/90‑1 (MQ=255)                                                                    aagCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGa         <  1:192499/90‑1 (MQ=255)                                                                      gCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAg       >  1:1223104/1‑90 (MQ=255)                                                                      gCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAg       >  1:141803/1‑90 (MQ=255)                                                                       cGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgc      <  2:1526020/90‑1 (MQ=255)                                                                       cGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgc      <  2:1198812/90‑1 (MQ=255)                                                                         tACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc    <  1:1562272/90‑1 (MQ=255)                                                                         tACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc    <  2:818749/90‑1 (MQ=255)                                                                          aCATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGcc   <  1:168548/90‑1 (MQ=255)                                                                           cATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCg  >  2:1448086/1‑90 (MQ=255)                                                                                gCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc    >  2:403278/1‑83 (MQ=255)                                                                                gCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc    <  1:403278/83‑1 (MQ=255)                                                                                  aGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc    >  1:941631/1‑81 (MQ=255)                                                                                  aGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc    <  2:941631/81‑1 (MQ=255)                                                                                      |                                                                               AACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCG  >  NZ_CP009273/139533‑139696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑