Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,659,576	T→C	F14S (TTC→TCC)	clcB →	voltage‑gated ClC‑type chloride channel ClcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,659,576	0	T	C	100.0%	110.3 / NA	35	F14S (TTC→TCC)	clcB	voltage‑gated ClC‑type chloride channel ClcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/17);  total (18/17)

TTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTTCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACTGGAG  >  NZ_CP009273/1659490‑1659661                                                                                       |                                                                                      ttttGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCa                                                                                    <  1:1049445/90‑1 (MQ=255)    tGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCg                                                                                 <  1:345758/90‑1 (MQ=255)                       gtgtTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAgtc                                                              >  2:1480127/1‑90 (MQ=255)                        tgtTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAgtcg                                                             <  1:1255632/90‑1 (MQ=255)                               ttGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTAt                                                      <  1:1028006/90‑1 (MQ=255)                               ttGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTAt                                                      <  1:369108/90‑1 (MQ=255)                                 gCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCtt                                                                          <  2:897919/68‑1 (MQ=255)                                 gCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCtt                                                                          >  1:897919/1‑68 (MQ=255)                                 gCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATtc                                                    >  2:659335/1‑90 (MQ=255)                                           gCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCtt                                          >  2:158006/1‑90 (MQ=255)                                                tGCATCATCTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTttgccg                                     >  2:1202646/1‑90 (MQ=255)                                                    tcatcTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTttgccgttgc                                 <  2:1048082/90‑1 (MQ=255)                                                    tcatcTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATCCTCGCGGCCTttgccgttgc                                 <  2:473229/90‑1 (MQ=255)                                                     catcTACACATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTttgccgttgcc                                >  2:1433255/1‑90 (MQ=255)                                                          acacATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGtt                           >  2:612179/1‑90 (MQ=255)                                                          acacATCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGtt                           >  2:948931/1‑90 (MQ=255)                                                           cacaTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGttt                          <  2:30599/90‑1 (MQ=255)                                                            acaTCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTc                         >  1:478847/1‑90 (MQ=255)                                                               tCTATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTc                      >  2:318510/1‑90 (MQ=255)                                                                 tATCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCAt                    >  2:542451/1‑90 (MQ=255)                                                                  aTCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAAc                                                                  <  2:978736/43‑1 (MQ=255)                                                                  aTCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAAc                                                                  >  1:978736/1‑43 (MQ=255)                                                                  aTCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATg                   >  2:309978/1‑90 (MQ=255)                                                                  aTCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATg                   >  1:432202/1‑90 (MQ=255)                                                                   tCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGc                  <  1:936999/90‑1 (MQ=255)                                                                   tCCGGATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGc                  <  1:158006/90‑1 (MQ=255)                                                                   gccGGATCGGCGCACTATGTCCCGCCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGc                  <  1:692336/89‑1 (MQ=255)                                                                       gATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTTCCGGGTTTCGTCATGCGAtg              >  1:3093/1‑90 (MQ=255)                                                                       gATCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGAtg              >  2:620409/1‑90 (MQ=255)                                                                         tCTGCGCACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGAtgct            <  2:1425135/90‑1 (MQ=255)                                                                            gcgcACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATgctgct         >  2:1547127/1‑90 (MQ=255)                                                                            gcgcACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATgctgct         >  1:220435/1‑90 (MQ=255)                                                                              gcACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTAc       <  2:1545495/90‑1 (MQ=255)                                                                               cACTATGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACt      <  1:659335/90‑1 (MQ=255)                                                                                   aTGTCCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACTGGAg  <  2:432202/90‑1 (MQ=255)                                                                                       |                                                                                      TTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTGCTCATGCATCATCTACACATCTATCCGGATCTGCGCACTATGTTCCACCGTCTGCTTATCGCAACAGTCGTCGGTATTCTCGCGGCCTTTGCCGTTGCCGGGTTTCGTCATGCGATGCTGCTACTGGAG  >  NZ_CP009273/1659490‑1659661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑