| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,673,542 | A→G | K287E (AAG→GAG) | ydgH → | DUF1471 family protein YdgH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,673,542 | 0 | A | G | 96.8% | 82.9 / NA | 31 | K287E (AAG→GAG) | ydgH | DUF1471 family protein YdgH |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (6/24); total (6/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGAT > NZ_CP009273/1673458‑1673616 | atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa < 1:1071814/90‑1 (MQ=255)atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa < 2:1512221/90‑1 (MQ=255)atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa < 2:1045479/90‑1 (MQ=255)atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa < 1:585036/90‑1 (MQ=255) ggTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAgg < 1:394500/90‑1 (MQ=255) ccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCt < 1:846391/90‑1 (MQ=255) ccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCt < 2:830181/90‑1 (MQ=255) ccGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCt < 2:1104588/90‑1 (MQ=255) cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa < 1:5984/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa < 2:920124/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa < 2:1319893/90‑1 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa > 2:257635/1‑89 (MQ=255) gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa < 1:257635/89‑1 (MQ=255) aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCt < 1:189110/83‑1 (MQ=255) aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCt > 2:189110/1‑83 (MQ=255) aCAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTAtt > 1:1057792/1‑90 (MQ=255) ctctGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa < 1:1364798/64‑1 (MQ=255) ctctGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa > 2:1364798/1‑64 (MQ=255) ggCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCAccagccag < 1:762506/90‑1 (MQ=255) cgacAACACGCCCAAATTCTCCTATAAGGTGGCGAAACGGGCGGGGGAGAAGGGTGATAATTATTCCCACATCCCCCGCCAGTGGCAGGa < 1:965054/87‑1 (MQ=255) aaCATGCCCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAAAAAGGTGCTAAGTATTCCAACACCACCCGCCAGTGGCAGGAACGt < 1:310168/90‑1 (MQ=255) aaCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGGGGAAAAAGGTGCTAAGTATTACCACATCCCCCGCCAGTGGCAGGAACGt < 1:450912/90‑1 (MQ=255) aaCATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGt < 2:557193/90‑1 (MQ=255) aaCATGACCGAAGTCTCCTATCAGGTTGCGAAACGGGCCGCGGAGAAAGGTGCTAAGTATTCCCACATCCCCCGCCAGTGGCAGGAACGt < 1:275432/90‑1 (MQ=255) aaCATCCCCGAACTCTCCTATCGGGTTGCCAAACGTGCCGCGGAGAAAGATGTTAATTATTACCAAATCACCCGCCAGTGGCAGGAACGt < 2:718398/90‑1 (MQ=255) tGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCCCCCGCCAGTGGCAGGAACGTGGta < 2:1057792/90‑1 (MQ=255) aCCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGtaat > 1:1549037/1‑90 (MQ=255) tATCAGTTTGCGAAACGTGCCGGGGAAAAGGGTGTTAATTTTTCCCACATCCCCCGCCAGTGGCAGGAACGTGGTAATAATCTGACCGTc < 1:370637/90‑1 (MQ=255) aGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGGAATAACCTGACCGTCAgcg > 1:86462/1‑90 (MQ=255) gtttGCAAAACGTGCCGGGGAGAAAGGTGATAATTATTACCCCATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcgc < 2:728064/88‑1 (MQ=255) gagAAAGGTGCGGAGAAGAAGGGTTTAAAATTTTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGAt < 1:158780/88‑1 (MQ=255) | ATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGAT > NZ_CP009273/1673458‑1673616 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |