| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,358,446 | Δ1 bp | coding (414/426 nt) | nudI → | nucleoside triphosphatase NudI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,358,444 | 0 | A | . | 94.1% | 60.6 / ‑3.3 | 17 | coding (412/426 nt) | nudI | nucleoside triphosphatase NudI |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (10/6); minor base G (1/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATA > NZ_CP009273/2358361‑2358526 | agTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGtct < 2:722054/90‑1 (MQ=255) aCTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACa > 1:258498/1‑90 (MQ=255) cTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGaagg > 1:518815/1‑75 (MQ=255) cTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGaagg < 2:518815/77‑3 (MQ=255) tACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAat > 2:409252/1‑90 (MQ=255) aCGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGaagg > 1:713415/1‑73 (MQ=255) aCGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGaagg < 2:713415/75‑3 (MQ=255) gtggATCTGGTGCATTATGATTTGACTGCCGCCACCCGAAAAACGTTACGTTTGGAAGGTCTTCTGTAACAATGGTGACAGCCATTACTg > 2:169133/4‑90 (MQ=255) gAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTgg > 1:340596/1‑90 (MQ=255) cTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGaagg < 1:612420/52‑3 (MQ=255) cTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGaagg > 2:612420/1‑50 (MQ=255) aTTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGaa > 1:47011/1‑90 (MQ=255) aTTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGaa < 1:783888/90‑1 (MQ=255) gATTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAAttt > 2:143430/1‑90 (MQ=255) aTTTGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTa > 1:238371/1‑90 (MQ=255) ttGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGAtt < 1:641469/79‑1 (MQ=255) ttGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGAtt > 2:641469/1‑79 (MQ=255) ttGAATGTCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGt > 2:189041/1‑90 (MQ=255) tCGCCACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAAc > 2:701073/1‑90 (MQ=255) ccACCCGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGaa < 1:378843/90‑1 (MQ=255) cGAAAAACGTTACGTTTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTccc < 2:340596/90‑1 (MQ=255) gttacgttTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATCCCCCATCCAGa > 2:364043/1‑90 (MQ=255) tacgttTG‑AAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATa < 2:689690/90‑1 (MQ=255) | AGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATA > NZ_CP009273/2358361‑2358526 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |