Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,898,828 (T)5→4 coding (130/1878 nt) bglF ← PTS beta‑glucoside transporter subunit IIABC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,898,8240T.95.8% 183.7 / 3.6 48coding (134/1878 nt)bglFPTS beta‑glucoside transporter subunit IIABC
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base . (27/19);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

TAACCGCCAGGAAGACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTT  >  NZ_CP009273/3898735‑3898909
                                                                                         |                                                                                     
tAACCGCCAGGAAGACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCt                                                                                       >  1:82859/1‑90 (MQ=255)
      ccAGGAAGACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCttttcag                                                                                 >  2:9153/1‑87 (MQ=255)
      ccAGGAAGACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCttttcag                                                                                 <  2:130150/90‑4 (MQ=255)
      ccAGGAAGACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCttttcag                                                                                 >  2:109829/1‑87 (MQ=255)
        aGGAAGACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTa                                                                              >  2:181305/1‑90 (MQ=255)
        aGGAAGACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTa                                                                              >  2:624114/1‑90 (MQ=255)
        aGGAAGACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTCTTCAGTa                                                                              >  1:748995/1‑90 (MQ=255)
          gAAGACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTAcc                                                                            <  2:698636/90‑1 (MQ=255)
             gACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACctct                                                                         >  1:326184/1‑90 (MQ=255)
                       acaTGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCgctt                                                               >  1:442614/1‑90 (MQ=255)
                              tACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTc                                                        >  1:283572/1‑90 (MQ=255)
                                  tATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATcc                                                    >  1:54218/1‑90 (MQ=255)
                                      accaccTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCttta                                                >  1:689750/1‑90 (MQ=255)
                                         accTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCtttaatt                                             >  1:538839/1‑90 (MQ=255)
                                          ccTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCttt                                                                                     <  1:680005/50‑1 (MQ=255)
                                          ccTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCttt                                                                                     >  2:680005/1‑50 (MQ=255)
                                          ccTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCtttaattt                                            >  1:554614/1‑90 (MQ=255)
                                            tGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCtttaatttaa                                          <  2:241381/90‑1 (MQ=255)
                                            tGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCtttaatttaa                                          <  2:772610/90‑1 (MQ=255)
                                              aaaCTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTaaaa                                        <  1:423149/90‑1 (MQ=255)
                                              aaaCTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTaaaa                                        <  1:341140/90‑1 (MQ=255)
                                                   ggCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTaa                                   >  2:779193/1‑90 (MQ=255)
                                                    gCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAAt                                  <  2:799880/90‑1 (MQ=255)
                                                    gCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAAt                                  >  1:794652/1‑90 (MQ=255)
                                                    gCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAAt                                  >  1:354542/1‑90 (MQ=255)
                                                         cGCTTTCCACTACCATAATTATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGt                             <  2:117075/90‑1 (MQ=255)
                                                         cGCTTTCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGt                             <  2:208233/90‑1 (MQ=255)
                                                             ttCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTgcg                         >  2:415768/1‑90 (MQ=255)
                                                              tCCACCACCATAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTgcgc                        <  1:162151/90‑1 (MQ=255)
                                                                cacccccaTAATAATACCTGTGCTCTTTTCAGTCCCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCaa                      <  2:538839/90‑1 (MQ=255)
                                                                caccaccaTAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCaa                      <  2:224069/90‑1 (MQ=255)
                                                                 accaccaTAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCaa                      <  2:815022/90‑1 (MQ=255)
                                                                 accaccaTAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCaa                      <  2:82859/90‑1 (MQ=255)
                                                                    accaTAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTgcgc                        <  1:454640/84‑1 (MQ=255)
                                                                    accaTAATAATACCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTgcgc                        >  2:454640/1‑84 (MQ=255)
                                                                       ataataataCCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCa               >  2:264126/1‑90 (MQ=255)
                                                                       ataataataCCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCa               >  1:398289/1‑90 (MQ=255)
                                                                       ataataataCCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCa               >  2:626445/1‑90 (MQ=255)
                                                                        taataataCCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAg              <  1:826330/90‑1 (MQ=255)
                                                                          ataataCCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGAc            <  1:9153/90‑1 (MQ=255)
                                                                             ataCCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTca         <  1:181305/90‑1 (MQ=255)
                                                                             ataCCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTca         <  1:571273/90‑1 (MQ=255)
                                                                              taCCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTcac        >  1:670381/1‑90 (MQ=255)
                                                                               aCCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTcgca       >  2:86682/1‑90 (MQ=255)
                                                                               aCCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTcaca       >  1:46104/1‑90 (MQ=255)
                                                                               aCCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTca         >  2:601623/1‑88 (MQ=255)
                                                                               aCCGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTca         <  1:601623/88‑1 (MQ=255)
                                                                                ccGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACaa      <  1:410966/90‑1 (MQ=255)
                                                                                ccGGGGGTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACaa      <  1:67676/90‑1 (MQ=255)
                                                                                  gggggTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATg    >  2:544229/1‑90 (MQ=255)
                                                                                  gggggTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATg    >  1:368906/1‑90 (MQ=255)
                                                                                  gggggTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATg    >  2:827275/1‑90 (MQ=255)
                                                                                  gggggTCTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATg    >  1:345196/1‑90 (MQ=255)
                                                                                    gggTCTTTTCAGTACCTCTGCTTGCGCTTTGGTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGtt  >  1:638278/1‑90 (MQ=255)
                                                                                         |                                                                                     
TAACCGCCAGGAAGACATCGGCCACATGGTTACCTATGACCACCTGAAACTGGCCACCGCTTTCCACCACCATAATAATACCGGGGGTCTTTTTCAGTACCTCTGCTTGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTT  >  NZ_CP009273/3898735‑3898909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: