Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	950,036	(T)5→4	intergenic (‑114/+292)	focA ← / ← ycaO	formate transporter FocA/30S ribosomal protein S12 methylthiotransferase accessory factor YcaO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	950,032	0	T	.	100.0%	32.4 / NA	9	intergenic (‑110/+296)	focA/ycaO	formate transporter FocA/30S ribosomal protein S12 methylthiotransferase accessory factor YcaO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (6/3);  total (6/3)

GGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTT  >  NZ_CP009273/949946‑950109                                                                                       |                                                                              ggCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGtttt                                                                            <  1:530889/90‑1 (MQ=255)   cgacgaGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGttttat                                                                          <  2:197051/90‑3 (MQ=255)   cgacgaGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGttttat                                                                          <  2:4904/90‑3 (MQ=255)           tACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGG‑TTTTATGCAAATAc                                                                 <  1:378379/90‑1 (MQ=255)                 cAAAGCAATATAGATGGGAAATTGATAAAAATAATATCCCGCCTGGCTTATAGGCCCGTAACTCGCCTGG‑TTTTATGCAAATACGGAGTa                                                           <  2:484656/90‑1 (MQ=255)                  aaaGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGG‑TTTTATGCAAATACGGAGTaa                                                          <  2:280175/90‑1 (MQ=255)                               tGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGG‑TTTTATGCAAATACGGAGTAAATATTTGATTATc                                             >  1:409768/1‑90 (MQ=255)                                  gaAATTGATATAGACCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGG‑TTTTATGCAAATACGGAGTAAATATTTGATTATCCaa                                          >  1:765245/1‑90 (MQ=255)                                          tataGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGG‑TTTTATGCAAATACGGAGTAAATATTTGATTATCCaaataaaaat                                  >  2:581901/1‑90 (MQ=255)                                            taGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGG‑TTTTATGCAAATACGGAGTAAATATTTGATTATCCaaataaaaataa                                >  2:637452/1‑90 (MQ=255)                                                         gCCTGGCTTATAGGCCCGTAACTCGCATGG‑TTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTa                   >  1:59490/1‑90 (MQ=255)                                                                          gTAACTCGCATGG‑TTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAAttt  >  1:547973/1‑90 (MQ=255)                                                                                       |                                                                              GGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTT  >  NZ_CP009273/949946‑950109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑