Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273108,7890TC62.5% ‑1.0 / 17.2 24S100G (AGC→GGC) zapDcell division protein ZapD
Reads supporting (aligned to +/- strand):  ref base T (5/4);  new base C (0/15);  total (5/19)
Fisher's exact test for biased strand distribution p-value = 2.96e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATT‑AATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTGGA  >  NZ_CP009273/108706‑108866
                                                                                    |                                                                             
gATGCTCGGTCGCTGACGCCCCAGACCAATCAAACGATCTTCACGCAGAAATTGCCCGATCCGCGGCGCGGAAATT‑AATACGCCCCccgc                                                                         <  2:345663/90‑1 (MQ=255)
        gccGCTGCCCCCCCAGACCAAACAAACGTCCTCCCCGCAAAAATTCCCCGATAGGCGGCGCGAAAATT‑AACCCCCCCCCCGCCGCTTTTa                                                                 <  2:420904/88‑1 (MQ=255)
           gCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATT‑AATACGCTCCCCGCCGCTTTTAAct                                                              >  1:683436/1‑90 (MQ=255)
                      agagCAATCAAACTATCATCCCAAAAAAATTGCCCGACGCGCGGCCCGAAAATT‑ATCTCGCTCCCCGCCGCTTTTAACTGCTGAATTAAt                                                   <  2:456277/90‑1 (MQ=255)
                      agagCAATCAAACGATCTTCCCGAAAAAATTCCCCGATCCGCGGCGCGAAAATT‑AATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAAt                                                   <  1:655643/90‑1 (MQ=255)
                      agagCAATCAAACGATCTTCACGCAAAAATTGCCCGATACGCGGCGCGGAAATT‑AATACGCTCCCCGCCGCTTTTAACTGCTGAATTAAt                                                   <  1:305020/90‑1 (MQ=255)
                      agagAAATCAAACGATCTTCCCGAAAAAATTCCCCGATACGCGGCGCGGAAATT‑AATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAAt                                                   <  2:568821/90‑1 (MQ=255)
                      agagAAATAAAACGATCTCCCCGAAAAAATTCCCCGATACGCGGCGCGAAAATT‑AATACCCCCCCCGCCGCTTTTAACTGCTGAATTAAt                                                   <  1:598824/90‑1 (MQ=255)
                        agCAATCAAACGATCTTCCCGCAAAAATTCCCCGATCCGCGGCGGGAAAATT‑AATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAATGc                                                 <  2:122701/90‑1 (MQ=255)
                        accAATAAAACGATCTCCCCGCAAAAATTCCCCGATACGCGGCGCGAAAATT‑AATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGc                                                 <  2:629200/88‑1 (MQ=255)
                         gCAAACAAACGATCTTCACGAAGAAATTCCCCGATCCGGGGCGCGGAAATT‑AATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCt                                                <  1:610713/90‑1 (MQ=255)
                            aTCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATT‑AATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCa                                             >  1:117952/1‑90 (MQ=255)
                            aTCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATT‑AATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCa                                             >  2:777463/1‑90 (MQ=255)
                                aaCGATCTCCACGAAAAAATGCCCCGATACGGGGCGCGAAAATT‑AATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATAc                                         <  2:827101/90‑1 (MQ=255)
                                 aCGTTCTTCCCGCAAAATTCGCCCGATCCGCGGCGCGAAAATT‑AATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACg                                        <  2:653057/90‑1 (MQ=255)
                                     tctcCACGCAAAATTTCCCCAATACGGGGCGCGAAAATT‑AATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGctc                                    <  2:804925/90‑1 (MQ=255)
                                      cTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATT‑AATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGctct                                   >  2:396054/1‑90 (MQ=255)
                                           cGCAAAAATTGCCCGATCCGCGGCGGGGAAATT‑AATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTcc                              <  1:389070/90‑1 (MQ=255)
                                               aaaaTTGCCCGATACGCGGCGCGGAAATT‑AATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGc                          <  1:188045/89‑1 (MQ=255)
                                                         gATACGCGGCGCGGAAATT‑AATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGcc                >  2:424464/1‑90 (MQ=255)
                                                              gcggcgcgaAAATA‑AATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATcc           <  1:583923/90‑1 (MQ=255)
                                                              gcggcg‑ggaAATTAAATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATcc           <  2:4877/90‑1 (MQ=255)
                                                                     gaaaaTT‑AATCCCCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTg    <  1:777463/88‑1 (MQ=255)
                                                                       aaaTT‑AATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTGGa  <  1:396054/90‑1 (MQ=255)
                                                                                    |                                                                             
GATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATT‑AATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAATCCAGGTCTGGA  >  NZ_CP009273/108706‑108866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑