| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 585,386 | 0 | A | G | 60.9% | 7.1 / 24.3 | 23 | L342P (CTC→CCC) | nfrA | bacteriophage adsorption protein NfrA |
| Reads supporting (aligned to +/- strand): ref base A (0/9); new base G (14/0); total (14/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.22e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.74e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAAC > NZ_CP009273/585300‑585459 | gCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCgggggggg > 1:437175/1‑90 (MQ=255) aTCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGgggggggggg > 1:136725/1‑90 (MQ=255) agCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTgcgc < 2:486956/90‑1 (MQ=255) agCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTgcgc < 1:544880/90‑1 (MQ=255) agCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTgcgc < 2:634766/90‑1 (MQ=255) tCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGAGGAGGTTTTGTGCcgcg > 2:769742/1‑90 (MQ=255) tGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGTTTTTCGCTGCGTCGGACt > 1:757363/1‑90 (MQ=255) tGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGGTTTTCTCTGcgtcggcct > 2:616209/1‑86 (MQ=255) aCGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGAcc < 1:51865/90‑1 (MQ=255) aCGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGAcc < 2:757063/90‑1 (MQ=255) cGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGTTTTTCGCCGCGTCGTTCTTACCTTCttt > 1:400875/1‑90 (MQ=255) gCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGTTTTTTGCTGCGGCGGACTTACCTTCtttt > 2:175948/1‑90 (MQ=255) cGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACa < 2:268371/90‑1 (MQ=255) tGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCa < 2:136725/90‑1 (MQ=255) cAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGTGGTTTTTGGGCTGCCGCGGATTTGCCTTTTTTTTATAGCACCg > 1:822848/1‑90 (MQ=255) cctcAAGCATTTCATTGGCGGGGGGGGTGGGGGGCAGATTTTTCTCTGCGGCGGTGGTACCTTTTTTTTAAAGCACCCGGAGGGgcgcgc > 1:617553/1‑90 (MQ=255) cctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGTTTTTTTGCTGCGTCGTACTGACCCTTTTTTTAAAACACCCGGAGCGGCccgc > 1:632116/1‑90 (MQ=255) cctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGTTTTTCGCGGCGTGGTACTGACCTTCTTTTTAAAACACCGGGAGCGgcgcgc > 1:91241/1‑90 (MQ=255) cctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGTTTTTTCGCTGCGGCGTACTGACCTTCTTTTTAAAACACCCGTAGCGgcgcgc > 1:797984/1‑90 (MQ=255) cctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTTGCTGCGTCGGACTGACCTTTTTTTTACAACACCGGGAGCGgcgcgc > 1:452672/1‑90 (MQ=255) cctcAAGCATTTCATTGGCGGGGGGGGGGGGGAGCAGGTTTTTCGCTGCGTCGTACTGACCTTTTTTTTAAAGCACCGGGAGCGgcgcgc > 1:221219/1‑90 (MQ=255) ctcAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGcc < 2:632116/90‑1 (MQ=255) cAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGTTTTTCGCTGCGCCGGACTGACCTTTTTTTTAAAACACCGGGAGGGGCGGGCcaa > 2:59308/1‑90 (MQ=255) aGCATTTCATTGGCGGGGGGGGGGGCGGGCAGTTTTTTCGCTGCGTCGTACTTACCTTCTTTTTACAGCACCGGGAGCGGCGCGCccaca > 1:284168/1‑90 (MQ=255) cATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCaacaac < 1:59308/90‑1 (MQ=255) | GCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAAC > NZ_CP009273/585300‑585459 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |