| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 760,187 | 0 | G | C | 64.7% | ‑0.0 / 10.1 | 17 | P184P (CCG→CCC) | sucD | succinate‑‑CoA ligase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (2/4); new base C (11/0); total (13/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTACACTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCG‑AAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAGCGC > NZ_CP009273/760098‑760276 | ggTACACTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCg < 2:428463/90‑1 (MQ=255) gACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGGGCCGCCCCCCGGCGCTaa > 2:352344/1‑90 (MQ=255) gACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGAGCCCAACCCCGGccctca > 2:634734/1‑88 (MQ=255) gACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGACCCGATCCCCGGCTCTaa > 1:39153/1‑90 (MQ=255) gACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGACCCCCTCCCCGGCCCTaa > 2:283485/1‑90 (MQ=255) gCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGAc < 1:600484/90‑1 (MQ=255) gCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGGGGCCCCACCCCCGGGGCTAACTTTTTTGAc > 2:139134/1‑90 (MQ=255) gTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGGCCCGCTCCCCGGGCCTCACTTTTTTGACAtt > 1:541181/1‑90 (MQ=255) tACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCCATCCCCGGCTCTAACTTTTTCGACATTTTCG‑AAAAGTTCGaaaaa > 1:255102/1‑90 (MQ=255) cGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCG‑AAATGTTCGAAAAAGa < 2:541181/90‑1 (MQ=255) tCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCCCTCCCCGGGCCTTACTTTTTTTACATTTTCGCAAAGGTTGGAAAAA‑ATCCGc > 2:809524/1‑90 (MQ=255) ggTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCCGGCTCTAACTTTTTTTACATTCTCG‑AAAAGTTTGGAAAAAAACCGCAg > 1:763343/1‑90 (MQ=255) ggTCAGTCGACCTGTGTCGGTATCGGCGGGGACCCCCTCCCCGGCTCTAACTTTTTTGACATTTTCG‑AAATGTTTTAAAAAAATCCGccg > 1:635203/1‑88 (MQ=255) cAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCG‑AAATGTTCGAAAAAGATCCGCAGAcc < 2:497598/90‑1 (MQ=255) gTCGACCTGTGTCGGTATCGGCGGTGACCCCATCCCGGGCTCTAACTTTTTCGACATTTTCG‑AAAAGTTTGAAAAAAATCCGCAGACCgg > 1:39011/1‑89 (MQ=255) gtgtCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTTTCGACATTTTCG‑AAATGTTCGAAAAAAATCCGCAGACCGAAGGGATCg > 1:297283/1‑90 (MQ=255) gtgtCGGTATCGGCGGTGACCCCATCCCCGGCTCTAAATTTATCGACATTCTCC‑AAAAGTTCGAAAAAAAACCCCAAACCCAAGCGGTCg > 1:752921/1‑90 (MQ=255) cggTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCG‑AAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTga < 1:800682/90‑1 (MQ=255) gggCTCTAACTTTATCGACATTCTCG‑AAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAgcgc < 2:720799/90‑1 (MQ=255) | GGTACACTGACCTATGAAGCGGTTAAACAGACCACGGATTACGGTTTCGGTCAGTCGACCTGTGTCGGTATCGGCGGTGACCCGATCCCGGGCTCTAACTTTATCGACATTCTCG‑AAATGTTCGAAAAAGATCCGCAGACCGAAGCGATCGTGATGATCGGTGAGATCGGCGGTAGCGC > NZ_CP009273/760098‑760276 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |