| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,167,328 | 0 | T | C | 66.7% | 8.2 / 10.1 | 15 | E698G (GAA→GGA) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base T (4/1); new base C (6/4); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAGGCCTAAATCTTTAAACTTGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTTCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGATACGTACCGGCCAGTTGGC > NZ_CP009273/1167245‑1167401 | gcagGCCTAAATCTTTAAACTTGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTTCCGCaa > 2:411184/1‑90 (MQ=255) tCTTTAAACTTGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTc < 2:343532/90‑1 (MQ=255) tttAAACTTGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTg > 1:510458/1‑90 (MQ=255) ttAAACTTGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGc < 1:327177/90‑1 (MQ=255) ttAAACTTGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGc > 1:529075/1‑90 (MQ=255) tGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTgg > 1:661882/1‑90 (MQ=255) tCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTg < 2:529075/90‑1 (MQ=255) ttGTGCGTACCGATCAGAATATTGTTTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATc < 2:327201/90‑1 (MQ=255) ttGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTTCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATc > 2:73672/1‑90 (MQ=255) tACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGa > 2:403615/1‑90 (MQ=255) aCCGATCAGAATATCGATTTTCCCTTCCGCCACTTCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGGTCATTTCGAt > 1:474133/1‑90 (MQ=255) aCCGATCAGAATATCGATTTTCCCTTCCGCCACTTCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGAt < 2:10471/90‑1 (MQ=255) tCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGATACGTACCGGCCAg > 1:685851/1‑90 (MQ=255) tttCCCTTCCGCCACTTCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGATACGTACCGGCCAGTTGGc > 1:741784/1‑90 (MQ=255) tttCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGATACGTACCGGCCAGTTGGc > 2:172302/1‑90 (MQ=255) | GCAGGCCTAAATCTTTAAACTTGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTTCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGATACGTACCGGCCAGTTGGC > NZ_CP009273/1167245‑1167401 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |