| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,276,249 | 0 | G | . | 63.6% | 0.8 / 15.7 | 11 | coding (930/3744 nt) | narG | nitrate reductase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (3/1); new base . (3/4); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGAC > NZ_CP009273/1276162‑1276327 | cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggc < 1:429112/90‑2 (MQ=255)cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggc < 1:462753/90‑2 (MQ=255) cGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAAtgct > 1:358609/1‑90 (MQ=255) aaCTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCt > 1:473823/1‑90 (MQ=255) gtgCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGAc > 1:266214/1‑90 (MQ=255) ccGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTc > 2:153851/1‑90 (MQ=255) cGAAACAGGGCACCGATGCGGCAATGGCCCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCCCGACAACCCAAGCCAGTATTTCAc < 2:758886/90‑1 (MQ=255) gggCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCAcc > 1:228276/1‑84 (MQ=255) gggCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCAcc < 2:228276/84‑1 (MQ=255) tGCGGCAATGGCGCTGGCGAT‑GTCCACGTAAGGCTGCGTAAATTCCCCCTCGACAACCAAAGCCAGTATTTCACCGACTATGTGCGTCGc < 2:473823/90‑1 (MQ=255) ggCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCCCCCCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGAc < 2:76942/90‑1 (MQ=255) tGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTc > 1:767521/1‑75 (MQ=255) tGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTc < 2:767521/75‑1 (MQ=255) | CACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGAC > NZ_CP009273/1276162‑1276327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |