| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,771,044 | 0 | A | G | 70.6% | 37.2 / 24.3 | 34 | K96E (AAA→GAA) | lldP | L‑lactate permease |
| Reads supporting (aligned to +/- strand): ref base A (7/3); new base G (15/9); total (22/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGTTTATGGTTTCTTCTACGGGTTGTGGCCCATCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGAAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGCAAATGCTGATCGTCGGTT > NZ_CP009273/3770964‑3771125 | tggtTTATGGTTTCTTCTACGGGTTGTGGCCCATCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGc < 1:257348/90‑1 (MQ=255) gtTTATGGTTTCTTCTACGGGTTGTGGCCCATCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGcca > 2:165992/1‑88 (MQ=255) tcttctACGGGTTGTGCCCCATCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATACGATCTCGGTGGAAACCGGGCCGTTTGAcatca > 1:384684/1‑90 (MQ=255) tctACGGGTTGTGGCCCATCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTc < 1:777029/90‑1 (MQ=255) ttGTGGCCCATCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGAAAACCGGGCAGTTTGACATCATTCGCTCGTCt > 2:828311/1‑90 (MQ=255) ttGTGGCCCATCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGAAAACCGGGCAGTTTGACATCATTCGCTCGTCt > 2:449104/1‑90 (MQ=255) aTCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCg > 1:377642/1‑90 (MQ=255) gcgTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATa < 1:252105/90‑1 (MQ=255) gcgTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATa < 2:705828/90‑1 (MQ=255) gcgTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATa < 2:472769/90‑1 (MQ=255) gcgTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGAAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATa < 1:502675/90‑1 (MQ=255) tCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGAAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTg > 2:744789/1‑90 (MQ=255) cATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAAccc > 1:278249/1‑86 (MQ=255) cATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAAccc < 2:278249/86‑1 (MQ=255) attattGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGAc > 1:569012/1‑90 (MQ=255) gCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGTAGTTTAACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGt > 2:759043/1‑90 (MQ=255) gCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGt < 1:49408/90‑1 (MQ=255) gCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGAAATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGt > 2:445871/1‑90 (MQ=255) gCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTCTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGt > 1:751867/1‑90 (MQ=255) gCAGCGGTGTTCGTCTATAAGATCTCGGTGAAAACCGGGc > 1:573339/1‑40 (MQ=255) gCAGCGGTGTTCGTCTATAAGATCTCGGTGAAAACCGGGc < 2:573339/40‑1 (MQ=255) gCAGCGGTGTTCGTCTATAAGATCTCGGTGAAAACCGGGCAGTTTGACATCATTCGc > 1:502898/1‑57 (MQ=255) gCAGCGGTGTTCGTCTATAAGATCTCGGTGAAAACCGGGCAGTTTGACATCATTCGc < 2:502898/57‑1 (MQ=255) gCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTg > 1:529409/1‑90 (MQ=255) gCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTg > 1:151018/1‑90 (MQ=255) gtTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCt > 2:522835/1‑60 (MQ=255) gtTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCt < 1:522835/60‑1 (MQ=255) gtTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGCAAAt > 1:31654/1‑90 (MQ=255) tataAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGt < 2:376581/75‑1 (MQ=255) tataAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGt > 1:376581/1‑75 (MQ=255) ataAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGCAAATGCTGAtcg > 1:805077/1‑90 (MQ=255) tctcGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGCAAATGCTGATCGTCGGtt > 1:769314/1‑90 (MQ=255) tctcGGTGAAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGCAAATGCTGATCGTCGGtt > 1:431602/1‑90 (MQ=255) tctcGGTGAAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGAAAATGCTGATCGTCGGtt > 1:618501/1‑90 (MQ=255) | TGGTTTATGGTTTCTTCTACGGGTTGTGGCCCATCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGAAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGCAAATGCTGATCGTCGGTT > NZ_CP009273/3770964‑3771125 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |