| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,057,873 | 0 | C | G | 64.7% | 37.0 / 38.2 | 34 | W418C (TGG→TGC) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base C (7/5); new base G (12/10); total (19/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAATGCC > NZ_CP009273/4057784‑4057962 | aTTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGc < 2:427124/90‑1 (MQ=255) aatCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAAt < 1:167652/90‑1 (MQ=255) cAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAg > 2:197845/1‑90 (MQ=255) cAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAg > 1:523622/1‑90 (MQ=255) aaTGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGc < 1:332064/90‑1 (MQ=255) tGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGCCAGATAAAAATGAGCTg > 2:197413/1‑90 (MQ=255) aaCCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGc > 2:368105/1‑90 (MQ=255) aaCCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGc > 1:677183/1‑90 (MQ=255) ccGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGCCAGATAAAAATGAGCTGACGCaa < 2:611181/90‑1 (MQ=255) gTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAAc < 1:774708/90‑1 (MQ=255) tttttCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAAcc > 1:59011/1‑90 (MQ=255) tCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGt < 1:710552/90‑1 (MQ=255) gAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGt > 1:824187/1‑90 (MQ=255) aaGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCtt < 2:59011/90‑1 (MQ=255) gAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTg > 1:115646/1‑90 (MQ=255) aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg > 2:570206/1‑90 (MQ=255) aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg > 1:14854/1‑90 (MQ=255) aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg > 1:442986/1‑90 (MQ=255) aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGCCAGATAAAAATGAGCTGACGCa < 1:348629/63‑1 (MQ=255) aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGCCAGATAAAAATGAGCTGACGCa > 2:348629/1‑63 (MQ=255) aTCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcg > 2:102730/1‑90 (MQ=255) tCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcgc < 1:368105/90‑1 (MQ=255) tCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTgcgc < 1:156702/90‑1 (MQ=255) ccATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGa < 1:288310/90‑1 (MQ=255) ccATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGa < 1:152569/90‑1 (MQ=255) gTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACg > 1:267097/1‑53 (MQ=255) gTCAATGCAGCAATAATTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACg < 2:267097/53‑1 (MQ=255) aataatTGCCAGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAAcc > 1:523714/1‑90 (MQ=255) aGTGCACAAGGGCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTc < 2:43106/90‑1 (MQ=255) aGTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTc < 2:104098/90‑1 (MQ=255) aGTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTc < 1:223110/90‑1 (MQ=255) gTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCa > 1:691555/1‑90 (MQ=255) gTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCa > 2:608253/1‑90 (MQ=255) gTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCa > 1:130925/1‑90 (MQ=255) aGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAAt > 1:84483/1‑90 (MQ=255) ccAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAATGcc > 1:500046/1‑90 (MQ=255) | ATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAATCCCATCGTCAATGCAGCAATAATTGCCAGTGCACAAGGCCAGATAAAAATGAGCTGACGCAAACCCTGTAATGTCGCATCGCTTTGCGCGATGTTGGGAACATAACCAATTTGCGTCAGCATAATGCC > NZ_CP009273/4057784‑4057962 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |