| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,736 | A→T | intergenic (+105/‑87) | kefC → / → folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,736 | 0 | A | T | 80.5% | 99.1 / 19.2 | 41 | intergenic (+105/‑87) | kefC/folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base A (2/6); new base T (22/11); total (24/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
CACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAT > NZ_CP009273/49651‑49818 | cacTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATtttt > 2:135023/1‑90 (MQ=255) ttACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTc > 2:195246/1‑90 (MQ=255) ttACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTc > 2:167616/1‑90 (MQ=255) aGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAAca < 1:227408/90‑1 (MQ=255) cTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatc < 2:50774/90‑1 (MQ=255) cTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatc < 2:137205/90‑1 (MQ=255) cTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatc < 1:397395/90‑1 (MQ=255) aaCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatcat > 1:774086/1‑90 (MQ=255) aaCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatcat > 1:827881/1‑90 (MQ=255) ttgtttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGc > 1:448350/1‑90 (MQ=255) tttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCAcc < 2:774086/90‑1 (MQ=255) cATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTcgacga < 1:7917/90‑1 (MQ=255) aTTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCgacgac > 1:674925/1‑90 (MQ=255) gcggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACg < 1:282341/90‑1 (MQ=255) gcggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACg < 1:237363/90‑1 (MQ=255) ggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCt > 2:383050/1‑90 (MQ=255) gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCtt < 2:76736/90‑1 (MQ=255) gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCtt < 1:13116/90‑1 (MQ=255) gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCtt < 2:81120/90‑1 (MQ=255) cgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCttt < 1:270543/90‑1 (MQ=255) gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTa < 1:808711/90‑1 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTAc < 1:573552/72‑1 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTAc > 2:573552/1‑72 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg > 1:637016/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg > 1:533104/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg > 1:306363/1‑90 (MQ=255) gagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGa > 1:450019/1‑90 (MQ=255) gagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGa > 1:400521/1‑90 (MQ=255) gTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAt > 1:761807/1‑90 (MQ=255) gTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAt > 2:63846/1‑90 (MQ=255) tGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAtt > 1:156189/1‑90 (MQ=255) ggACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAttt > 2:104234/1‑90 (MQ=255) tCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAttttttt > 2:355360/1‑90 (MQ=255) tCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAttttttt > 2:458436/1‑90 (MQ=255) tCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAttttttt > 1:529506/1‑90 (MQ=255) tCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAttttttt > 2:7496/1‑90 (MQ=255) gcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCgg > 1:132352/1‑90 (MQ=255) gAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaa < 2:544084/90‑1 (MQ=255) gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaa < 1:167616/90‑1 (MQ=255) atatAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAt > 1:669630/1‑90 (MQ=255) atatAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAt < 2:183830/90‑1 (MQ=255) | CACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAAT > NZ_CP009273/49651‑49818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |