| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,941,604 | A→C | G10G (GGT→GGG) | ruvC ← | crossover junction endodeoxyribonuclease RuvC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,941,604 | 0 | A | C | 93.3% | 40.7 / ‑4.8 | 15 | G10G (GGT→GGG) | ruvC | crossover junction endodeoxyribonuclease RuvC |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (8/6); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACC > NZ_CP009273/1941517‑1941686 | atcCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAccc > 2:168211/1‑90 (MQ=255)atcCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAccc > 1:622315/1‑90 (MQ=255) tcCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAcccc < 2:378939/90‑1 (MQ=255) ttGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCa > 1:300295/1‑90 (MQ=255) ttGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCa > 2:481012/1‑90 (MQ=255) tGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCaa < 2:26118/90‑1 (MQ=255) ggATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCga > 2:252877/1‑90 (MQ=255) cAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCAc < 2:300295/90‑1 (MQ=255) aGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGtc < 2:152998/90‑1 (MQ=255) gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtt > 1:807001/1‑90 (MQ=255) gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtt > 1:429911/1‑90 (MQ=255) gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtt > 2:487697/1‑90 (MQ=255) gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtt > 2:501165/1‑90 (MQ=255) acctacctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGt < 1:579278/90‑1 (MQ=255) tacctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTa > 1:568072/1‑90 (MQ=255) gCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGc > 1:149058/1‑90 (MQ=255) aCGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGAc < 2:738307/90‑1 (MQ=255) cgcgcgACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGAcc < 1:481012/90‑1 (MQ=255) | ATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACC > NZ_CP009273/1941517‑1941686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |