| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 231,042 | A→C | G76G (GGT→GGG) | gloB ← | hydroxyacylglutathione hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 231,042 | 0 | A | C | 76.9% | 59.6 / 12.8 | 26 | G76G (GGT→GGG) | gloB | hydroxyacylglutathione hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base C (13/7); total (16/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 | |||||||||||
TACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTGAGAAATA > NZ_CP009273/230958‑231124 | tACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATa > 2:733217/1‑90 (MQ=255) acacTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATac < 1:716695/90‑1 (MQ=255) acacTAAATTCATGCCCCAAAACCAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATac < 2:61701/90‑1 (MQ=255) tAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATAcacca > 1:838654/1‑90 (MQ=255) aaaTTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATAcaccac > 2:245657/1‑90 (MQ=255) aaaTTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATAcaccac > 2:579033/1‑90 (MQ=255) gCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTtgtg > 2:227111/1‑90 (MQ=255) cAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGaaa > 2:358015/1‑90 (MQ=255) gCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTccacc < 1:94374/90‑1 (MQ=255) tGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTAcgccgc < 1:103981/90‑1 (MQ=255) gACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACgccgcc < 1:551311/90‑1 (MQ=255) gACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACgccgcc < 1:733217/90‑1 (MQ=255) aCCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACg > 1:79285/1‑90 (MQ=255) cTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTg > 1:545787/1‑90 (MQ=255) tgttgtTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATc < 1:12526/90‑1 (MQ=255) tgttCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCgtg > 1:782241/1‑90 (MQ=255) tCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCgtggtg > 1:472933/1‑90 (MQ=255) tCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCgtggtg > 1:36598/1‑90 (MQ=255) tCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCgtggtg > 1:821529/1‑90 (MQ=255) cccTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGa > 1:735397/1‑90 (MQ=255) cTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGAtg > 2:387473/1‑90 (MQ=255) cTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGAtg > 2:820619/1‑90 (MQ=255) tgtgTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATggtgggtg < 1:245657/90‑1 (MQ=255) tgtgTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATggtgggtg < 1:466638/90‑1 (MQ=255) tgtCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTgag < 2:188068/90‑1 (MQ=255) ctTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTGAGAAata > 2:216780/1‑90 (MQ=255) | TACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTGAGAAATA > NZ_CP009273/230958‑231124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |