Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,131,004	A→G	intergenic (‑280/‑379)	wza ← / → yegH	polysaccharide export protein Wza/TerC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,131,004	0	A	G	89.7%	71.4 / 3.0	29	intergenic (‑280/‑379)	wza/yegH	polysaccharide export protein Wza/TerC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base G (11/15);  total (11/18)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01

TGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACG  >  NZ_CP009273/2130917‑2131087                                                                                        |                                                                                    tGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGgaa                                                                                   >  1:809085/1‑90 (MQ=255)     tttttAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTaa                                                                               <  1:314914/90‑1 (MQ=255)                cAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAatat                                                                    <  1:517772/90‑1 (MQ=255)                     cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                               <  2:830810/90‑1 (MQ=255)                     cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                               <  2:307822/90‑1 (MQ=255)                     cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                               <  2:289304/90‑1 (MQ=255)                     cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                               <  1:709925/90‑1 (MQ=255)                     cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGt                                                               <  1:120098/90‑1 (MQ=255)                      acTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTg                                                              <  1:397340/90‑1 (MQ=255)                         gATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACa                                                           >  2:281034/1‑90 (MQ=255)                                tttAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGt                                                    <  1:709466/90‑1 (MQ=255)                                 ttAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTa                                                   >  1:623933/1‑90 (MQ=255)                                 ttAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTa                                                   <  2:553120/90‑1 (MQ=255)                                   aaTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAAt                                                 >  1:387566/1‑90 (MQ=255)                                       tttCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGaa                                             >  1:402330/1‑90 (MQ=255)                                            cacCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTg                                        <  1:435775/90‑1 (MQ=255)                                                 tctcAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCttt                                   <  1:657660/90‑1 (MQ=255)                                                   tcAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTcc                                 <  2:272394/90‑1 (MQ=255)                                                      gCATGCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCaat                              <  1:310767/90‑1 (MQ=255)                                                      gCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCgat                              <  1:512455/90‑1 (MQ=255)                                                          gCAGTCGTTGATGAGGAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATg                          >  1:196221/1‑90 (MQ=255)                                                          gCAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATg                          >  2:380766/1‑90 (MQ=255)                                                           cAGTCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGc                         >  2:534048/1‑90 (MQ=255)                                                              tCGTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCagg                      >  2:758806/1‑90 (MQ=255)                                                                gTTGATGAGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCaggag                    <  2:551398/90‑1 (MQ=255)                                                                  tgatgaGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCaggagca                  >  2:495975/1‑88 (MQ=255)                                                                  tgatgaGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAggagga                  >  2:791212/1‑90 (MQ=255)                                                                    atgaGAAAGGGTTATTACGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAg                <  1:534048/90‑1 (MQ=255)                                                                                 attaCGGGAATTAACTTCCGAATATAAGGTGACATTGTGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAAc   <  1:319267/90‑1 (MQ=255)                                                                                  ttaCGGGAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACg  >  1:178193/1‑90 (MQ=255)                                                                                        |                                                                                    TGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACG  >  NZ_CP009273/2130917‑2131087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑