Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,833,262	T→G	V127G (GTC→GGC)	ascF →	PTS cellobiose/arbutin/salicin transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,833,262	0	T	G	100.0%	112.5 / NA	35	V127G (GTC→GGC)	ascF	PTS cellobiose/arbutin/salicin transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/19);  total (16/19)

CGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGTCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATT  >  NZ_CP009273/2833175‑2833341                                                                                        |                                                                                cGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCa                                                                               >  2:633327/1‑90 (MQ=255)  gCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCaa                                                                              <  1:633327/90‑1 (MQ=255)    tACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAAc                                                                            <  1:229446/90‑1 (MQ=255)     aCGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAAct                                                                           >  2:698382/1‑90 (MQ=255)          gCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTgg                                                                      >  1:499220/1‑90 (MQ=255)               ggTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATg                                                                 <  2:543158/90‑1 (MQ=255)               ggTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATg                                                                 <  2:252463/90‑1 (MQ=255)               ggTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATg                                                                 <  1:727463/90‑1 (MQ=255)                    ggggATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATccc                                                            >  2:728392/1‑89 (MQ=255)                    ggggATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCt                                                            >  1:394908/1‑90 (MQ=255)                      ggATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCg                                                          <  2:216051/90‑1 (MQ=255)                         tCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCgaga                                                       >  2:288806/1‑90 (MQ=255)                          ccTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGGTCGATGGGCAAACTGCTGGCAATGATCCTCGAGAt                                                      >  2:704574/1‑90 (MQ=255)                          ccTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGAt                                                      >  2:58979/1‑90 (MQ=255)                                tGCGCTGATCGGCACCATGTCCCCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAgcgg                                                <  2:753404/90‑1 (MQ=255)                                      gATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGGGCt                                          >  1:209146/1‑90 (MQ=255)                                       aTCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTg                                         <  1:675254/90‑1 (MQ=255)                                          ggCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACa                                      <  1:704574/90‑1 (MQ=255)                                           gCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGGGCTGACaa                                     >  2:297150/1‑90 (MQ=255)                                                aTGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGc                                           <  1:35770/79‑1 (MQ=255)                                                aTGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGc                                           >  2:35770/1‑79 (MQ=255)                                                aTGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGa                                >  2:275353/1‑90 (MQ=255)                                                     aCCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGcc                           <  2:394908/90‑1 (MQ=255)                                                     aCCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGcc                           >  1:777187/1‑90 (MQ=255)                                                     aCCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGcc                           >  1:728094/1‑90 (MQ=255)                                                     aCCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGcc                           <  1:330719/90‑1 (MQ=255)                                                     aCCGCTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGcc                           <  1:230053/90‑1 (MQ=255)                                                         cTGATCCCGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGAcc                       <  1:447595/90‑1 (MQ=255)                                                                cGGCGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCa                >  2:88024/1‑90 (MQ=255)                                                                   cGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTc             <  1:286028/90‑1 (MQ=255)                                                                   cGATTATCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTc             <  1:275353/90‑1 (MQ=255)                                                                         tCGGCGGATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAAtg       <  2:733133/90‑1 (MQ=255)                                                                              ggATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGAtt  <  1:620218/90‑1 (MQ=255)                                                                               gATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGc                            <  2:479363/63‑1 (MQ=255)                                                                               gATCGATGGGCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGc                            >  1:479363/1‑63 (MQ=255)                                                                                        |                                                                                CGCTACGTCGCATTGGTGCGGGGATCCTCGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGTCAAACTGCTGGCAATGATCCTCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATT  >  NZ_CP009273/2833175‑2833341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑