| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,882,413 | A→C | V349G (GTT→GGT) | cysI ← | assimilatory sulfite reductase (NADPH) hemoprotein subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,882,413 | 0 | A | C | 93.3% | 90.7 / ‑1.5 | 30 | V349G (GTT→GGT) | cysI | assimilatory sulfite reductase (NADPH) hemoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base C (12/16); total (12/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GGCCGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACT > NZ_CP009273/2882324‑2882493 | ggCCGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTaa > 1:649079/1‑90 (MQ=255) ccGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAccc < 2:183725/90‑1 (MQ=255) ccGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAccc < 2:23503/90‑1 (MQ=255) ccGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAccc < 1:711425/90‑1 (MQ=255) ccGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAccc < 1:663795/90‑1 (MQ=255) ccGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAccc > 1:632392/1‑90 (MQ=255) ccGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAccc > 1:363299/1‑90 (MQ=255) ccGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAccc > 1:289513/1‑90 (MQ=255) cGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAcccc < 2:399468/90‑1 (MQ=255) gTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAg > 2:726410/1‑90 (MQ=255) cAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAAt > 1:135112/1‑90 (MQ=255) gCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAccc < 2:678529/80‑1 (MQ=255) gCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAccc > 1:678529/1‑80 (MQ=255) gACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGAt > 2:65294/1‑90 (MQ=255) cgcCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGcc < 1:83035/90‑1 (MQ=255) aTAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTcc < 1:164987/90‑1 (MQ=255) aaTCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCgg < 1:451018/90‑1 (MQ=255) cAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCACGTCCGGTGa < 1:635241/90‑1 (MQ=255) aGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAAc < 1:701848/90‑1 (MQ=255) aGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAAc < 2:180756/90‑1 (MQ=255) aGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAAc < 2:553371/90‑1 (MQ=255) aGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCACCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAAc < 1:270222/90‑1 (MQ=255) ggATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACt > 2:592213/1‑90 (MQ=255) aTGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAAc < 2:836331/87‑1 (MQ=255) aTGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAAc > 1:836331/1‑87 (MQ=255) gCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCata > 2:401515/1‑90 (MQ=255) cGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCatat > 1:758937/1‑90 (MQ=255) ttttCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACgg > 2:300862/1‑90 (MQ=255) tCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATc < 2:441051/90‑1 (MQ=255) aTAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGt > 1:646299/1‑90 (MQ=255) aaaCAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTc < 2:135112/90‑1 (MQ=255) aaaCAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTc < 2:646299/90‑1 (MQ=255) aCAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCaa < 1:421490/90‑1 (MQ=255) gCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGAc > 2:484060/1‑75 (MQ=255) gCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGAc < 1:484060/75‑1 (MQ=255) cGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGcc < 1:741204/51‑1 (MQ=255) cGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGcc > 2:741204/1‑51 (MQ=255) aGGTGCCAGTTATCATCAATGCCCTTCCCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATc < 2:506562/90‑1 (MQ=255) tATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGAc < 2:632392/90‑1 (MQ=255) aTGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACt > 1:182511/1‑90 (MQ=255) ttACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGAc < 2:788438/76‑1 (MQ=255) | GGCCGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACT > NZ_CP009273/2882324‑2882493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |