| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,613,222 | C→G | R238P (CGC→CCC) | lplA ← | lipoate‑‑protein ligase LplA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,613,222 | 0 | C | G | 87.5% | 42.0 / 1.1 | 16 | R238P (CGC→CCC) | lplA | lipoate‑‑protein ligase LplA |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base G (12/2); total (14/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01 | |||||||||||
GTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATG > NZ_CP009273/4613136‑4613304 | gTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTgggggg > 1:459653/1‑90 (MQ=255) aaGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGC‑AAGGTTTCGGCGAAGTTTg > 2:160940/1‑90 (MQ=255) aaGCGTTCATCCAGCAGATGCGAGAAGGCCGGAGCCTGACCGAAGTTCCATTCCCCGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGttt > 2:492303/1‑90 (MQ=255) aGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTg > 1:759122/1‑90 (MQ=255) gCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTgg > 1:596529/1‑90 (MQ=255) ttCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCaa > 1:107503/1‑90 (MQ=255) gATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGttt < 2:272040/90‑1 (MQ=255) aTGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCgggg > 1:461378/1‑90 (MQ=255) tGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGga > 1:449237/1‑90 (MQ=255) tGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGga > 2:93287/1‑90 (MQ=255) cTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTc > 2:427690/1‑90 (MQ=255) gACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCg > 1:273596/1‑90 (MQ=255) cccAGCTACTCTGGCGGGCAAAGGGTTCGGCGAAGTTTGGCAAGTTTGGCGTTTTGTTCGGGGAGATGAGTTCCGCGTCGACGGGCTCGc > 2:462502/1‑90 (MQ=255) cAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCa > 2:421067/1‑40 (MQ=39) cAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCa < 1:421067/40‑1 (MQ=39) gCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATa > 2:409936/1‑90 (MQ=255) aCTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATg > 2:374439/1‑90 (MQ=255) | GTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATG > NZ_CP009273/4613136‑4613304 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |