| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,431,536 | T→C | E1169G (GAA→GGA) | nifJ ← | pyruvate:ferredoxin (flavodoxin) oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,431,536 | 0 | T | C | 88.9% | 40.7 / ‑0.6 | 18 | E1169G (GAA→GGA) | nifJ | pyruvate:ferredoxin (flavodoxin) oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (9/7); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
AAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCAC > NZ_CP009273/1431458‑1431618 | aaGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCg > 2:577137/1‑90 (MQ=255) aGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCgg < 1:488770/90‑1 (MQ=255) aGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCCTACCCAGAGCATTTAATCGGTGCTGCTCTTTTCCGCTTTTCCGGCCATTtgtg < 2:428763/90‑1 (MQ=255) cTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCa > 1:512440/1‑90 (MQ=255) ccAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATc < 2:136146/90‑1 (MQ=255) ccAGAGCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGAGTTTTTGCAAATCAGCTGCAGCATc < 1:577137/90‑1 (MQ=255) agagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCtt > 2:704168/1‑90 (MQ=255) agagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCtt > 1:96286/1‑90 (MQ=255) agagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCtt < 1:463930/90‑1 (MQ=255) gagCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCttt > 2:210923/1‑90 (MQ=255) gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGc < 2:24287/39‑1 (MQ=39) gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGc > 1:24287/1‑39 (MQ=39) gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATc > 1:579146/1‑87 (MQ=255) gagCATTTAATCGGTGTTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATc < 2:579146/87‑1 (MQ=255) gagCATTTAATCGGCGTTGATTTTTCCCGCTTTTCCTGCCATTTGTGCCCGGAAGGCATAGCGGTTTTGCAAATCATCTGCAGCATCgtt > 2:570142/1‑90 (MQ=255) gtgtTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCt > 1:83876/1‑90 (MQ=255) gtTGCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGc > 1:457568/1‑90 (MQ=255) gCTTTTTCCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCAc < 2:363324/90‑1 (MQ=255) | AAGCCCGAACAGTCGTCCGGGCTTTTTTTTTAGAATTGGATAATCCTTATCCAGAGCATTTAATCGGTGTTGCTTTTTTCCGCTTTTCCGGCCATTTGTGCCAGGAAGTCATAGCGTTTTTGCAAATCAGCTGCAGCATCTTTCCATAACTGTTCTGCCAC > NZ_CP009273/1431458‑1431618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |