| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,505,430 | A→G | K391E (AAA→GAA) | ydcR → | PLP‑dependent aminotransferase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,505,430 | 0 | A | G | 89.5% | 40.5 / ‑0.2 | 19 | K391E (AAA→GAA) | ydcR | PLP‑dependent aminotransferase family protein |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (8/9); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01 | |||||||||||
TTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCAC > NZ_CP009273/1505350‑1505514 | ttCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATtcatc < 2:58259/90‑1 (MQ=255) tcgCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATaa > 2:464826/1‑90 (MQ=255) ccTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGa < 2:601163/90‑1 (MQ=255) gCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGtt < 1:704609/90‑1 (MQ=255) gCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTtctc < 1:775860/90‑1 (MQ=255) gCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTtctc < 2:429305/90‑1 (MQ=255) cGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGTGCTcccc > 1:13222/1‑90 (MQ=255) tGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGa > 1:666145/1‑90 (MQ=255) gCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATtca > 1:22122/1‑47 (MQ=255) gCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATtca < 2:22122/47‑1 (MQ=255) gCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTcatcat < 2:362989/51‑1 (MQ=255) gCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTcatcat > 1:362989/1‑51 (MQ=255) caCTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATg > 2:778484/1‑90 (MQ=255) ctgcTTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAGCCGTTAGATGCCGGc < 2:98458/90‑1 (MQ=255) ctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGTTTCCTTTCTTTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGc < 1:485325/90‑1 (MQ=255) ctgcctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGc > 2:61353/1‑90 (MQ=255) tgcctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGcc > 2:485325/1‑90 (MQ=255) cctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTg > 1:32245/1‑90 (MQ=255) aaGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCAc > 2:828490/1‑90 (MQ=255) | TTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCAC > NZ_CP009273/1505350‑1505514 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |