Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,524,815	T→C	intergenic (‑54/‑28)	BW25113_RS25600 ← / → BW25113_RS07655	protein YncO/ISAs1 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,524,815	0	T	C	100.0%	42.7 / NA	14	intergenic (‑54/‑28)	BW25113_RS25600/BW25113_RS07655	protein YncO/ISAs1 family transposase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/9);  total (5/9)

GGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCC  >  NZ_CP009273/1524729‑1524883                                                                                       |                                                                     gggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGaacaac                                                                   >  1:666178/1‑90 (MQ=255) gggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGGGATCAAAATTTAACCTTTGGaacaac                                                                   >  2:462705/1‑90 (MQ=255)      aGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTaaaa                                                              >  2:740022/1‑90 (MQ=255)         tCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGa                                                           <  2:585323/90‑1 (MQ=255)           aTGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATa                                                         >  2:672313/1‑90 (MQ=255)                  ttGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAgg                                                  >  1:702427/1‑90 (MQ=255)                  ttGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAgg                                                  <  2:205815/90‑1 (MQ=255)                                              tGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCtt                      <  1:750991/90‑1 (MQ=255)                                              tGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCtt                      <  2:536301/90‑1 (MQ=255)                                               gCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTg                     <  2:642437/90‑1 (MQ=255)                                               gCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTg                     <  2:702427/90‑1 (MQ=255)                                                          tACAGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCa          >  2:365210/1‑90 (MQ=255)                                                             aGGTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTg       <  2:59680/90‑1 (MQ=255)                                                              ggTGATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGa      <  1:365210/90‑1 (MQ=255)                                                                 gATCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAccc   >  2:113597/1‑90 (MQ=255)                                                                  aTCAAAATTTAACCTTTGGAACAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAcccc  <  1:740022/90‑1 (MQ=255)                                                                                       |                                                                     GGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCC  >  NZ_CP009273/1524729‑1524883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑