| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 115,628 | A→C | T137P (ACG→CCG) | ampD → | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 115,628 | 0 | A | C | 87.1% | 66.9 / 1.7 | 31 | T137P (ACG→CCG) | ampD | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Reads supporting (aligned to +/- strand): ref base A (3/1); new base C (17/10); total (20/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01 | |||||||||||
AATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATC‑GGAAAACCGATC > NZ_CP009273/115544‑115712 | aaTGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCgg > 2:71180/1‑90 (MQ=255)aaTGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCgg < 1:783650/90‑1 (MQ=255) tGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGc < 2:836679/90‑1 (MQ=255) tGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGc < 1:312266/90‑1 (MQ=255) aTTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCcgggcc > 2:605164/1‑87 (MQ=255) ttGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTgattgatt > 2:822351/1‑90 (MQ=255) ttGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTgattgatt < 1:279956/90‑1 (MQ=255) gggATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGc < 1:523485/90‑1 (MQ=255) tGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGattgattg < 1:507940/78‑1 (MQ=255) tGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGattgattg > 2:507940/1‑78 (MQ=255) cACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCAc < 1:83672/62‑1 (MQ=255) cACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCAc > 2:83672/1‑62 (MQ=255) cTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCCCGGGCGCTGATTGAATGCTATCCGGATATCTCTAAAAAAATGACg > 2:46351/1‑90 (MQ=255) ggCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGGTCCGCGGGCACTTATTGATTGCTATCCGGGTATCGCTAAAAACATGACggg > 2:398522/1‑90 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 1:71180/90‑1 (MQ=255) tataCCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCAt < 1:605164/90‑1 (MQ=255) taCCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCAt < 1:540121/88‑1 (MQ=255) taCCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCAt > 2:540121/1‑88 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGat > 1:129731/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAAAATGACGGGCCATTGTGat > 1:617526/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAAGACGGGGCCTTGTGat > 2:164319/1‑90 (MQ=255) aTGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGata > 1:296703/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGGCCTTGTGGTATTTCGCCGGGTT‑GGaaa > 2:145520/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATC‑GGaaa > 1:64918/1‑90 (MQ=255) cTTGCGGCGGTTCCCCGGGGCCTGATTGATTGCTATCCGGGTATCGCTAAAAACATGAGGGGGCATTGTGATATTTCGCCGGGTC‑GGgaa > 2:709579/1‑90 (MQ=255) cTTGCGGCGGTTACGCGGGGACTGATTGATTGCTATCCGGATATCGCTAAAAAAATGACGGGCCATTGTGATATTGCGCCGGATC‑GGaaa > 1:628553/1‑90 (MQ=255) cTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAt < 2:137739/56‑1 (MQ=255) cTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACAt > 1:137739/1‑56 (MQ=255) tGCGGCGGTTCCGCGGGCACTGATTGATTTCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGGTC‑GGAAAAc > 2:533707/1‑90 (MQ=255) tGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTTCGCCGGATC‑GGaaaaa > 1:825711/1‑89 (MQ=255) ggTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAAAATGACGGGCCATTGTGGTATTTCGCCGGATCGGGAAAAccaacc > 2:607016/1‑86 (MQ=255) | AATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATC‑GGAAAACCGATC > NZ_CP009273/115544‑115712 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |