| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,531,275 | G→A | L98L (CTG→TTG) | narW ← | nitrate reductase molybdenum cofactor assembly chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,531,275 | 0 | G | A | 89.2% | 112.1 / 2.7 | 37 | L98L (CTG→TTG) | narW | nitrate reductase molybdenum cofactor assembly chaperone |
| Reads supporting (aligned to +/- strand): ref base G (4/0); new base A (18/15); total (22/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
ATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAA > NZ_CP009273/1531191‑1531359 | aTCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCa > 1:375325/1‑90 (MQ=255)aTCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCa > 2:550512/1‑90 (MQ=255)aTCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCa > 2:82726/1‑90 (MQ=255) tCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAg > 2:628680/1‑90 (MQ=255) tcttTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCg < 1:496664/90‑1 (MQ=255) tttCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGAc < 2:569986/90‑1 (MQ=255) tttCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGAc < 1:667067/90‑1 (MQ=255) tCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCt < 1:545686/90‑1 (MQ=255) tCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCt < 1:628680/90‑1 (MQ=255) gTACGCTTAAATTCTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGTCAATCCAACTGCAGGCCGACCTTTTCATACTCCGCcagca < 2:519426/90‑1 (MQ=255) gTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCcagca > 1:406247/1‑90 (MQ=255) gTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCcagca > 2:808856/1‑90 (MQ=255) aCGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAgg > 2:83686/1‑90 (MQ=255) aCGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAgg > 1:613158/1‑90 (MQ=255) cGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCcagcggtc < 1:77551/90‑5 (MQ=255) cGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGt > 2:402111/1‑90 (MQ=255) ttAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGGcca > 2:602574/1‑90 (MQ=255) aaaTACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTccacc < 1:82726/90‑1 (MQ=255) aTACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCAt < 2:150309/90‑1 (MQ=255) cTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGc > 2:423530/1‑90 (MQ=255) cTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGc > 1:491316/1‑90 (MQ=255) cTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGc > 1:171136/1‑90 (MQ=255) cTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGc > 2:747458/1‑90 (MQ=255) tCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGcc < 1:760893/90‑1 (MQ=255) ccAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCt > 2:813749/1‑90 (MQ=255) cAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCcgcg > 1:409546/1‑90 (MQ=255) aGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGa > 2:714409/1‑90 (MQ=255) aGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGa > 2:85110/1‑90 (MQ=255) aGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATa > 2:252118/1‑50 (MQ=255) aGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATa < 1:252118/50‑1 (MQ=255) aaTCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACt < 1:402111/90‑1 (MQ=255) gggCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGc > 1:785545/1‑90 (MQ=255) ggCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCa < 1:101999/90‑1 (MQ=255) ttCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAAc < 1:490045/90‑1 (MQ=255) aTCCAACTGCAGGCCGACCTTTTCATACTCCGCcagca > 2:158727/1‑38 (MQ=39) aTCCAACTGCAGGCCGACCTTTTCATACTCCGCcagca < 1:158727/38‑1 (MQ=255) aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGaa > 1:722918/1‑90 (MQ=255) | ATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAA > NZ_CP009273/1531191‑1531359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |