| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,558,439 | A→G | Y518H (TAT→CAT) | dosP ← | oxygen‑sensing cyclic‑di‑GMP phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,558,439 | 0 | A | G | 94.7% | 52.9 / ‑3.9 | 19 | Y518H (TAT→CAT) | dosP | oxygen‑sensing cyclic‑di‑GMP phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (7/11); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 | |||||||||||
TGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGTAA > NZ_CP009273/1558353‑1558519 | tGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATc < 1:259280/90‑1 (MQ=255) gcgcCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATcc > 2:464403/1‑90 (MQ=255) caaaacCCAACCCCTTTTTTCCCTTTTCTTTCATCGCCGGGCTGAAGACCTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCcattg < 2:405655/88‑1 (MQ=255) cTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCGGGGCTGAAGACCTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCcattg < 1:659778/90‑1 (MQ=255) aCCAACCGCTTTTTTCCCTTTTCTTTCATCGCCGGGCTGAAAAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCcattgcattg < 2:566846/90‑1 (MQ=255) aCGCTTTTTTCCCTTTTCTTTCATCGCCGGGCTAAAAAACTCCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGc < 1:525472/90‑1 (MQ=255) aCGCTTTTTTCCCTTTTCGTTCATCGCCGGGCTGAAAACCTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGc < 2:708699/90‑1 (MQ=255) aTTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAg > 1:293455/1‑90 (MQ=255) aTTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAg > 1:667557/1‑90 (MQ=255) ttCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTa > 2:74676/1‑90 (MQ=255) caccgcgGGGCTGAAGACCTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgcg < 1:741254/87‑1 (MQ=255) caccGCCGGGTTAAAAAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgcg < 1:776612/87‑1 (MQ=255) tCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGt > 1:806116/1‑90 (MQ=255) tCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGt > 1:657522/1‑90 (MQ=255) ttGAAGACCTCCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTAccc < 2:806116/89‑1 (MQ=255) gaagaaCTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAg > 2:141035/1‑90 (MQ=255) gCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGAt < 2:360354/90‑1 (MQ=255) tCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGACGCCAATACTCAAgg < 1:69758/90‑1 (MQ=255) tGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCTAATACTCAAGGTaa > 2:644050/1‑90 (MQ=255) | TGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGTAA > NZ_CP009273/1558353‑1558519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |