| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 139,618 | A→G | E118G (GAA→GGA) | yadG → | ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 139,618 | 0 | A | G | 83.9% | 62.9 / 9.5 | 31 | E118G (GAA→GGA) | yadG | ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base G (16/10); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01 | |||||||||||
TTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATG > NZ_CP009273/139530‑139703 | ttCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGaa < 2:675096/90‑1 (MQ=255) aaCCCGTTTGAAACCGTGCAGCAAATTGTTGTGAATCCGGCAGGGTACCACGGCGCGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg < 2:365990/90‑1 (MQ=255) aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg < 2:434375/90‑1 (MQ=255) aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg < 2:149957/90‑1 (MQ=255) gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc > 2:712306/1‑90 (MQ=255) gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc > 1:794523/1‑90 (MQ=255) gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTc > 1:102785/1‑90 (MQ=255) gcagcaAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGa < 1:272739/90‑1 (MQ=255) gcaAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCt < 2:279357/90‑1 (MQ=255) aaTTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATg < 2:794523/90‑1 (MQ=255) gtgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGaaaa > 2:70147/1‑90 (MQ=255) tCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCaa < 2:361670/90‑1 (MQ=255) aggcaggGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCaacg > 2:158965/1‑90 (MQ=255) gggTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGt < 1:14666/90‑1 (MQ=255) ctacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGcgcg < 1:139487/90‑1 (MQ=255) gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt > 2:758731/1‑90 (MQ=255) gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt > 2:497639/1‑90 (MQ=255) gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt > 1:435092/1‑90 (MQ=255) gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAg < 2:517433/38‑1 (MQ=255) gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAg > 1:517433/1‑38 (MQ=255) gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa > 2:38875/1‑90 (MQ=255) gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa > 1:427588/1‑90 (MQ=255) gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa > 2:603490/1‑90 (MQ=255) gTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTa > 2:143852/1‑90 (MQ=255) gcAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCg > 2:231242/1‑90 (MQ=255) gTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcg < 1:65806/90‑1 (MQ=255) tCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGtt > 2:256249/1‑90 (MQ=255) ccGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGttt < 1:266277/90‑1 (MQ=255) gCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTaa > 2:135680/1‑90 (MQ=255) gCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTaa > 1:632800/1‑90 (MQ=255) gCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTaa > 1:840104/1‑90 (MQ=255) aGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATg < 1:542432/90‑1 (MQ=255) | TTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAATG > NZ_CP009273/139530‑139703 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |