Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,818,956 T→G K80T (AAA→ACA)  ves ← environmental stress‑induced protein Ves

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,818,9560TG75.0% 17.5 / 3.9 12K80T (AAA→ACA) vesenvironmental stress‑induced protein Ves
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (2/7);  total (3/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01

TGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCAC  >  NZ_CP009273/1818870‑1819020
                                                                                      |                                                                
tgatgTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGC‑AAGGCAAAAGGCTGGAACGGTGTTaa                                                              >  1:730566/1‑90 (MQ=255)
     ttGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTa                                                          <  1:27172/90‑1 (MQ=255)
     ttGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTGTTAAGGTa                                                          <  1:176288/90‑1 (MQ=255)
              aTCGACATCTGCCCTGCCGTCAGTTTCGCCTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTGTTAAGGTATGGTTAAAg                                                 <  1:727831/90‑1 (MQ=255)
                        gccctgccGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTGTTAAGGTATGGTTAAAGCGGTCTgcgc                                       <  1:221708/90‑1 (MQ=255)
                            tgccGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTGTTAAGGTATGGTTAAAGCGGTCTGCGCTTTc                                   >  2:260436/1‑90 (MQ=255)
                                          cTTTTACCACCTGGTCCGCTGC‑AAGGCAAAAGGCTGGAACGGTGTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTcgcc                    <  2:730566/90‑1 (MQ=255)
                                                   ccTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCa            >  2:478099/1‑90 (MQ=255)
                                                      ggTCCGCTGCAAAGGCAAAAGGCTGGAACGGTGTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCa         <  2:241811/90‑1 (MQ=255)
                                                             tGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCAc  <  2:707341/90‑1 (MQ=255)
                                                             tGCAAAGGCAAAAGGCTGGAACGGTGTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCAc  <  1:260436/90‑1 (MQ=255)
                                                             tGCAAAGGCAAAAGGCTGGAACGGTGTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCAc  <  2:150895/90‑1 (MQ=255)
                                                                                      |                                                                
TGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCAC  >  NZ_CP009273/1818870‑1819020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑