Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	799,325	0	G	T	55.6%	0.8 / 12.8	9	V123L (GTG→TTG)	ybhJ	hydratase
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base T (5/0);  total (5/4)
Fisher's exact test for biased strand distribution p-value = 7.94e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCG  >  NZ_CP009273/799241‑799382                                                                                     |                                                          tcactcTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCt                                                      <  1:81717/90‑1 (MQ=255)   actcTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTcc                                                    >  2:267226/1‑90 (MQ=255)   actcTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGGCTcc                                                    >  2:526600/1‑90 (MQ=255)                   acagcTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTcatc                                    <  1:565162/86‑1 (MQ=255)                       cTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTcatccatc                                <  1:267226/90‑1 (MQ=255)                                 tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGGTTTTTTTGGCCCCCCCTATTGTGGGCCTCCCTCAATATATggg                      >  1:652082/1‑88 (MQ=255)                                 tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCTCCCCCTATTTCGGGCATCCCTCAatataattt                      >  2:565162/1‑86 (MQ=255)                                   atgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTg                    <  2:259143/90‑1 (MQ=255)                                                     tATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCCCATATTTCGGGCATCCCTCAATATATTCGGGGGATGATGGGAggggggg  >  2:416882/1‑88 (MQ=255)                                                                                     |                                                          TCACTCTGCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCG  >  NZ_CP009273/799241‑799382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑