| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,663,765 | 0 | T | A | 61.5% | 0.7 / 12.3 | 13 | I29F (ATT→TTT) | ynfL | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base A (8/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATACACTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCG‑AAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAAT > NZ_CP009273/1663678‑1663843 | aTACACTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGCG‑AAaaat > 2:955606/1‑90 (MQ=255) acacTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCG‑AAATATTc < 1:657601/90‑1 (MQ=255) cTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTTCG‑GAAAAttttgg > 1:793808/1‑86 (MQ=255) cTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGGG‑GAAAATTCAgg > 1:799829/1‑90 (MQ=255) cTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGCG‑GAAAATTTAgg > 1:21480/1‑90 (MQ=255) gCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAAAAATTCAggg > 2:829904/1‑89 (MQ=255) gCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGGG‑GAAAATTTAGGCg > 1:107810/1‑90 (MQ=255) gCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGCG‑AAAAAATTAGGCg > 2:706959/1‑90 (MQ=255) ggTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGGG‑AAAAATTTAGGGGggcggc > 2:480967/1‑90 (MQ=255) ccAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCG‑AAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGctct < 1:694045/90‑1 (MQ=255) tgttgCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCG‑AAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCgca < 2:713665/90‑1 (MQ=255) aGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCG‑AAAAATTTAGGCGGGCAGCGGCGCGCCCCAAAAGCAGCTCTTCCccaacagcaaca > 2:112047/1‑90 (MQ=255) cTTAGCGGCGGTTGCG‑AAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGa < 1:483814/90‑1 (MQ=255) cggTTGCG‑AAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAat < 1:925819/90‑1 (MQ=255) | ATACACTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCG‑AAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAAT > NZ_CP009273/1663678‑1663843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |