| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,357 | 0 | A | T | 60.0% | 7.0 / 21.6 | 20 | intergenic (+47/‑56) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base A (1/7); new base T (12/0); total (13/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATT > NZ_CP009273/2380278‑2380442 | ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACATGCACATTTTTTTTTTCCTcc > 2:124207/1‑89 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 1:238941/90‑1 (MQ=255) taACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACAATTTTTTTTTTCTCATCTGttt > 2:755812/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCGGAAAAAAAGACAAACACAATTTTTTTTTTCTCATCtttttttaa > 1:430430/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACATGCACAATTTTTTTTTCCTCATCtttttttaa > 2:923759/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGAAAAGCACAATTTTTTTTTTCTCATCtttttttaa > 1:345764/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAAAAAAAAAGACAAGAACAATTTTTTTTTTCTCCTCtttttttaa > 1:115031/1‑87 (MQ=255) ccaacaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAAAAAAAGCGGCATGCACATTTGATTTTCCCTCATCTGTTTTAAGATGCGaaa < 1:260491/89‑1 (MQ=255) acaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACAt < 1:927322/90‑1 (MQ=255) acaGTAAATAATAAAAATTTCTCGAGGCGGGGGCAGAAAAAAAAAAAAGCACAATTTTTTTTTTCCCCTCTTTTTTTAAAAGCGaaaaaa > 1:424756/1‑87 (MQ=255) aGTAAATAATAAAAATTTCTCGAGGCGGGGGCCGAAAAAAAGAAAAGCACATTTTTTTTTTCCTCCTCTGTTTTTATATTGGAAAAATGa > 1:787787/1‑90 (MQ=255) ataaAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTAAGATGCGAAAAATGAAAATTAAc > 2:192813/1‑90 (MQ=255) ataaAAATTTCTCGAGGCGGGGCCCGAAAAAACGACAAGCACATTTTTTTTTTCCTCATCTGTTTTTAGAAGCGAAAAAAGAAAATCaaa > 2:31533/1‑89 (MQ=255) aaaaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACgga < 1:220719/90‑1 (MQ=255) ttCTCGAGGCGGGGCCCGAAAAAAAGACATGCACATTTTTTTTTTTCTCCTCTTTTTTTAAATGCGAAAAAAGAAAATTAAAGGAGGaaa > 1:68544/1‑90 (MQ=255) ttCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACATTTTTTTTTTTCTCCTCTTTTTTTAAATGGGGAAAAAGAAAATTAAAAGAGGaaa > 1:32955/1‑90 (MQ=255) gAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaataa < 1:172197/90‑1 (MQ=255) tCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAAt < 1:930189/90‑1 (MQ=255) gAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGAtt > 1:913660/1‑90 (MQ=255) gACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTAttt < 2:828762/90‑1 (MQ=255) ttttattttCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAAtt < 2:873211/90‑1 (MQ=255) | GGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATT > NZ_CP009273/2380278‑2380442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |