| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 228,191 | 0 | A | G | 55.2% | 11.8 / 28.2 | 29 | S195G (AGC→GGC) | yafD | endonuclease/exonuclease/phosphatase family protein |
| Reads supporting (aligned to +/- strand): ref base A (5/8); new base G (16/0); total (21/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.03e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGA > NZ_CP009273/228102‑228261 | aTGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGa < 1:842077/90‑1 (MQ=255) gTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCCCCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGc < 1:916508/90‑1 (MQ=255) gTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGGTTTAATTCCTGGGGc > 1:133148/1‑90 (MQ=255) gTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGGTTTAATGCCTGGGGc > 1:790820/1‑90 (MQ=255) gTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGc < 1:58722/90‑1 (MQ=255) gTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTAAATCCCGGGGCCGGAgct > 2:384809/1‑88 (MQ=255) aaacAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGATTTTAATGGCTCGGGCCGGCGAAgg > 2:795408/4‑90 (MQ=255) aaGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTTATTACTGGGGCCGGAGaaag > 2:457490/1‑88 (MQ=255) aaGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTAATTCCTGGGGGCGTGGAAgg > 2:720591/1‑90 (MQ=255) aaGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGGTTTAATGCCTCGAGGCGCGGAAgg > 1:138554/1‑90 (MQ=255) aaGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGGTTTAATACCTGGGGCCGGGGAAgg > 1:218119/1‑90 (MQ=255) gTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGATTTTAATGCCTGGGGGCGCAGGAGGATGaa > 1:905243/1‑90 (MQ=255) gTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGAATTTAATTCCTTGGGGCGTGGAAGGAGGaa > 2:832123/1‑90 (MQ=255) gTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGaa < 2:627566/90‑1 (MQ=255) gTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGaa < 1:941664/90‑1 (MQ=255) aCTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGGTTTTATGCCTGGGGGCGCAGGAGGAGGaagaa > 1:915756/1‑87 (MQ=255) cTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGGATTTAATTCCTTGGGGCGGAGAAGGAGGAACGCGGtat > 2:505307/1‑90 (MQ=255) gATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTTAATGCCTGGGGGCGGAGAAGGATGAAAGCGGTTTATTGCttt > 2:25291/1‑90 (MQ=255) caccacAGCGGCCCGGTCATTATGGCGGGGGGGTTTAATGCCTGGAGCCGGAGAAAGAAGAAAGCGGTTTATCGCTTTGCGCGGGgaaag > 2:740684/1‑88 (MQ=255) caccacAGCGGCCCGGTCATTATGGCGGGGGATTTTAATGCCTGGGGCCGGAGAAGGGAGAAAGCGGTATATCGCTTTGCGCGGGgaaag > 2:759934/1‑88 (MQ=255) cacaGCGGCCCGGTCATTATGGCGGGGGGTTTTAAAGCCTGGAGCCGGAGAAGGAGGAAAGCGTTATATTGCTTTTCGCGGGGAAAGTCg > 1:270271/1‑90 (MQ=255) caGCGGCCCGGTCATTATGGCGGGGGATTTTAATTCCTTGGGCCGGGGAAGGGGGAAAGCGGTTTATTGCTTTTTGCGGGGAAAGTCGCt > 1:357048/1‑90 (MQ=255) caGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCt < 2:930406/90‑1 (MQ=255) caGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCt < 2:133148/90‑1 (MQ=255) gCGGCCCGGTCATTATGGCGGGAGATTTTAATGCCTGGAGCCGTAGAAGGATGAACGCGGTATATCGCTTTGCGCGGGGAAAGTtgctgc > 2:170565/1‑90 (MQ=255) ccGGTCATTATGGCGGGAGATTTCAATGCCTGGGGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGGAATGTCGCTGCGcccg > 2:141876/1‑88 (MQ=255) ttATGGCGGGAGATTTTAATTCCTGGGGGCGCAGAAGGATGAACGCGGTTTATCGCTTTTCGCGGGGAAAGTGGCTGCGCCAGGGGCggt > 2:434535/1‑88 (MQ=255) aTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGtttt < 2:138554/90‑1 (MQ=255) cGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGGGCGTTTTACCg > 2:55833/1‑90 (MQ=255) gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCga < 2:270271/90‑1 (MQ=255) | ATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGA > NZ_CP009273/228102‑228261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |