| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,419,801 | 0 | T | G | 58.8% | 7.0 / 15.2 | 17 | L41V (TTG→GTG) | cycA | D‑serine/D‑alanine/glycine transporter |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (10/0); total (13/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGT > NZ_CP009273/4419713‑4419889 | tCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgtt < 2:736069/90‑1 (MQ=255)tCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgtt < 1:513273/90‑1 (MQ=255)tCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgtt < 2:119385/90‑1 (MQ=255)tCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgtt < 2:124767/90‑1 (MQ=255) gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATgggg < 1:166703/90‑1 (MQ=255) cTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGt < 1:388908/90‑1 (MQ=255) tCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCaaa < 2:290045/90‑1 (MQ=255) cGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGTCGGGGGTGTTTTTTGGGGCTGGCaaaa > 1:100740/1‑90 (MQ=255) tACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGTGGTGGCGTTGGTGCGGGGTGGTTTTTGGGGGCTGGCGAAACgg > 2:194639/1‑89 (MQ=255) tACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGTCCATGGGGAGGGGGGTGGTTGTTGGGGGTGGCAGAACGa > 2:593157/1‑90 (MQ=255) tACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGGCCCTTGGTACGGGGGTGTTTTTTGGGGCTGGCAAAAAGa > 1:288229/1‑90 (MQ=255) gACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGAATAGCCTTGCCGGGGCGTCgg > 1:885705/1‑89 (MQ=255) gACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGGCTGGCAAAACGATTAGCCCTGCCGGGCCGTCGa > 1:156418/1‑90 (MQ=255) gACATATTCAGCTTATTGCCATTGGCGGAGCCTATTGCAGGGGGGTGTTTATGGGGGGCGGTAAAAAAAATAGGCTTACCGGGCCggcaa > 1:237223/1‑86 (MQ=255) cAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCAttttt > 2:478998/1‑89 (MQ=255) aTTGCCATTGGCGGGGGCCTTTGGCCGGGGGTGTTTTTTGGGGCCCGCAAAACGATTTGCCCTGGCGGGGCGCCTGTCATTTTTGttttt > 1:948407/1‑88 (MQ=255) aTTGGCGGTGCCCTTGGTACGGGGGTGTTTTTGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATTATc > 2:907256/1‑90 (MQ=255) tGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAt < 1:96082/90‑1 (MQ=255) gTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGtt > 2:708756/1‑90 (MQ=255) ttGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGc > 2:480631/1‑90 (MQ=255) tACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCtt > 2:691402/1‑90 (MQ=255) gttgttTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGt < 2:77861/90‑1 (MQ=255) | TCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGT > NZ_CP009273/4419713‑4419889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |