| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 711,081 | 0 | A | C | 66.7% | 6.1 / 11.2 | 15 | T72P (ACC→CCC) | BW25113_RS03560 | lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/10); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCC > NZ_CP009273/710994‑711169 | aCCAGAGGATAAACGGGTAGCTTTGGGGAAAATTTTCCCCCTTCCCGGTGAAAGAAAAATTGCCGTGGTGATAACTTCCCTGATAATccc < 1:918621/90‑1 (MQ=255) ccAGAGGATTAAGGGGTAGCATTGCGGTAAATATTGCCCTTTTCCGGTGTAAGAAAAATTGCCGTGGTAATAACTTACCTGATAATcccc < 1:368431/90‑1 (MQ=255) gagGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACccgcc > 1:904884/1‑90 (MQ=255) gTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGt > 2:990632/1‑90 (MQ=255) gTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGt > 2:18924/1‑90 (MQ=255) gaaaaTATTCCCCCTTTCCGGTGTAAGAAAAATTGCCGTGGTAATAACTTACCTAATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAAc < 1:169597/88‑1 (MQ=255) gccccTTCCCGGTGTAAAACAAATTGCGGGGGTAAAAACTTCCCTAATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGaa < 1:917035/87‑1 (MQ=255) tcccGGTGAAAAAAAAATTGCGGTGGTAATAACTTACCTAATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAacttact < 1:84745/88‑1 (MQ=255) cGGTGAAAGAAAATTTCCGGGGGAGAAAACTTCCCTGATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTAt < 1:534118/90‑1 (MQ=255) gtgaAAGAAAAATTGCGGTGGTAATAACTTCCCTGAAAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCt < 2:809840/90‑1 (MQ=255) aaaaaTTGCCGGGGTAATAACTTACCTGATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCataat < 2:372646/88‑1 (MQ=255) aCAAATTGCCGTGGTGATAACTTCCCTGATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCataat < 2:387354/90‑1 (MQ=255) cGTGGTAATAACTTCCCTGATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGca < 2:127770/90‑1 (MQ=255) ggggtaATAACTTCCCTAACAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGcac < 1:431520/88‑1 (MQ=255) gATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGaa > 1:963873/1‑90 (MQ=255) tcccTGATAATCCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCg < 2:864257/88‑1 (MQ=255) tACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCg > 1:201937/1‑90 (MQ=255) aataCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAAt > 1:690979/1‑90 (MQ=255) aataCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAAt > 1:589772/1‑90 (MQ=255) taCCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATcc > 1:651988/1‑90 (MQ=255) | ACCAGAGGATTAACGGGTAGCATTGCGGTAAATATTGCACCTTTCCGGTGTAAGACAAATTGCCGTGGTGATAACTTACCTGATAATACCCGCCGCTGGCAGTTGTCAGGCGGGAACGAAAAAGAACTTACTTATCTTCATAATATGTCAGCACAAGAAAAGGTCGGTTTAAATCC > NZ_CP009273/710994‑711169 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |