| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,377,967 | 0 | C | A | 56.2% | 12.7 / 26.1 | 16 | L84F (TTG→TTT) | ycjW | LacI family DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (6/1); new base A (0/9); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACC > NZ_CP009273/1377894‑1378044 | cttctGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGaaaa < 2:321181/90‑1 (MQ=255) ttGTGCGCGGTTTGAAATATCACGTCAAACCCTTCTTCTTCAGTTTGGGCGGTGATGGCATGAAAAACTTCAGAAAAAAACGGATTcccc < 1:742094/90‑1 (MQ=255) gtgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACccgc > 1:217899/1‑90 (MQ=255) aCGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAg > 2:678128/1‑90 (MQ=255) tGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTg > 1:971135/1‑90 (MQ=255) tCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAAc > 2:3005/1‑90 (MQ=255) cACGTCAAAACTTTCTTCTTCAGCTTGGGCGGTGATGGAAAGAAAACCTTCAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACg < 1:877855/90‑1 (MQ=255) ccGTCAAAACCTTTTTCTTCAGCTTTGGGGGTGATGGCATGAAAAACTTCAGAGAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACgg < 2:256374/89‑1 (MQ=255) aCGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGCCGAACgg > 1:865980/1‑90 (MQ=255) gTCGAAACCTTCTTCTTCAGCTTGGGGGGTGATGGCATGAAAAACTAAAAAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGt < 1:678128/90‑1 (MQ=255) gTCAAACCCTTTTTCTTCAGTTTTGGGGGTGATGGCATGAAAAACTTCAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGt < 2:835691/90‑1 (MQ=255) gTCAAAACTTTCTTCTTCAGTTTGGGGGGTGATGGAATAAAAAACTTAAAAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGt < 1:810665/90‑1 (MQ=255) gAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGa > 1:262630/1‑90 (MQ=255) ctttcttcttcAGCTTGGGGGGTGAGGGCAGAAAAAACTTAAAAAAAAAACGGTTTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATc < 1:852098/88‑1 (MQ=255) ccTTCTTCTTCAGCTTTGGCGGTGATGGCATGAAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATc < 2:217899/90‑1 (MQ=255) ggTGAGGGCAGGAAAAACTTCAGAAAACAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAAcc < 2:1010787/90‑1 (MQ=255) | CTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACC > NZ_CP009273/1377894‑1378044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |