| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,341 | 0 | G | A | 65.4% | 8.8 / 17.2 | 26 | intergenic (+31/‑72) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base G (0/9); new base A (17/0); total (17/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.20e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.01e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTG‑TTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGAT > NZ_CP009273/2380258‑2380423 | aaaTGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACAt < 2:310996/90‑1 (MQ=255) aaTGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATg < 1:1045060/90‑1 (MQ=255) gCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGcaca < 1:966710/90‑1 (MQ=255) tAGTGCATGGTGTTATAAATTTATTATAAAATAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATCCACattttatt < 1:605031/90‑1 (MQ=255) aTGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGAAAAGCACaattttttttttct > 1:183605/1‑86 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 2:806919/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 1:522876/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 2:141720/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 2:114973/90‑1 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCAGAAAAAACGACAAGAAAATTTTATTTTTCCTTTTCTG‑TTTTTAg > 1:512627/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACAAGAACATTTTATTTTTTCTCCTCTG‑tttttaa > 1:1031404/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCATCtt‑tttttaa > 2:1084658/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGGACATTTTTTTTTTCCTCATCTT‑TTTTTAg > 2:700585/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACAATTTTTTTTTTCTCATCTG‑TTTTTAg > 2:835294/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAACAAAAGAAAATTTTATTTTTTCTCAGCTG‑GTTTTAg > 1:979837/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCtt‑tttttaa > 2:432432/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACAATTTATTTTTCCTCATCTG‑tttttaa > 2:446295/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACATGCACATTTTATTTTTCCTCCtttt‑tttttaa > 2:483802/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACAATTTTTTTTTTCTCCTCTG‑tttttaa > 1:314224/1‑87 (MQ=255) tGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTG‑TTTTTAGAt > 2:106107/1‑90 (MQ=255) caacaGTAAATAATAAAAATTTCTCGAGGCGGGTTCAGAAAAAAAAACAAGATCAATTTTTTTTTTCTCCTCTGTTTTTAAAATGGGaaa > 1:633039/1‑90 (MQ=255) aaaTAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCACATTTTTTTTTTCCCCCTCTT‑TTTTTAAAAGCGGAAAAAGaaaa > 2:240837/1‑89 (MQ=255) taataaAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCATCTT‑TTTTTAAATGCGAAAAAAGaaaatta > 1:69546/1‑86 (MQ=255) ataaAAATTTCTCGAGGCGGGTCCCGAAAAAACGACATGCACATTTTTTTTTTCCCCCTCTG‑TTTTTAGATGCGAAAAATGAAAATCAAc > 2:281878/1‑90 (MQ=255) ataaAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAGCACATTTTTTTTTTCCTCATCTT‑TTTTTTGATGGGAAAAAAGAAAATTAAc > 1:132677/1‑90 (MQ=255) aGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTG‑TTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGAt < 1:206830/90‑1 (MQ=255) | AAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTG‑TTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGAT > NZ_CP009273/2380258‑2380423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |