| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,505,251 | 0 | A | C | 60.0% | 1.2 / 12.0 | 15 | V312G (GTG→GGG) | mntH | Nramp family divalent metal transporter |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/9); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACC > NZ_CP009273/2505163‑2505319 | cATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATcaccac > 2:133434/1‑90 (MQ=255) gACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCCCCCCCTGCCCCGc < 2:433011/90‑1 (MQ=255) aaaaaaTGACGGCAACAGGGTGACTGTACGCCCCCCCCACAGGGGGATATAAAAGCGAATGAAGCCCTGCATCCCCCCCTGCCCCGCCAg < 2:804141/87‑1 (MQ=255) ggCAACAGGGTGCCTGACCGCCCCCCCCCCGGGGGGATAAAAAAGCAAAGGAACCCCTGCACCCCCCCCGCCCCCGCCAGTGTCCccacc < 1:133434/90‑1 (MQ=255) cAACATGGTGACTGTACGCCGCACCCCCAGCGGGATATAAAAGCAAATGAAGCCCGGCATCACCCCCCGCCCCGCCAGTGTCCCcaccac < 2:357108/90‑1 (MQ=255) aCTGTACGACGCCCCCACAGCGGGATATAAAAGCGAATGAAGCCCTGCATCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGAc < 2:282595/90‑1 (MQ=255) gTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGc > 2:163363/1‑90 (MQ=255) cccccACAGGGGGATATAAAAGCGAATGAAGCCCTGCATCCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCgcagc < 2:30676/88‑1 (MQ=255) cACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCgcagc > 2:160525/1‑90 (MQ=255) cACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCgcagc > 2:201183/1‑90 (MQ=255) cccACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCaa > 2:609020/1‑90 (MQ=255) gaatatAAAAGCAAATGACCCCCGGCACCCCCCCCTCCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTa < 1:235781/88‑1 (MQ=255) gCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGAc > 1:914983/1‑90 (MQ=255) gCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCACAGCAACCAGGCTTAATCCAAAGAc > 2:602700/1‑90 (MQ=255) caaaTGAACCCCTGCAACCCCCCCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGAcc < 2:703628/88‑1 (MQ=255) cGAATGAACCCCGGCATCCCCCCCTCCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGAcc < 2:161148/90‑1 (MQ=255) | CATCAGAATGACAATAAATGACGGCAACATGGTGACTGTACGACGCACCCACAGCGGGATATGAAAGCGAATGAAGCCCTGCATCACCACCTGCCCCGCCAGTGTCCCCACCACCGTTGAAGACAGCCCCGCAGCAACCAGGCTTAATCCAAAGACC > NZ_CP009273/2505163‑2505319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |