Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,738,4480AC63.6% 7.8 / 30.5 34V84G (GTG→GGG) trmDtRNA (guanosine(37)‑N1)‑methyltransferase TrmD
Reads supporting (aligned to +/- strand):  ref base A (9/3);  new base C (0/21);  total (9/25)
Fisher's exact test for biased strand distribution p-value = 5.70e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-06
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCG  >  NZ_CP009273/2738359‑2738537
                                                                                         |                                                                                         
gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                           >  2:671316/1‑90 (MQ=255)
gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                           >  2:657064/1‑90 (MQ=255)
      acacCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTg                                                                                     <  1:460622/90‑1 (MQ=255)
                 aaTTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCAccc                                                                          >  1:20419/1‑90 (MQ=255)
                      ttGATTCGTTGCCAGTTCGCTGACCCCCGCTTGATCACGCTTGCGTCCTTGTGGTGAAAGATAAATCCCCTTTGCCCCTCCCCCcgccgc                                                                     <  1:572751/90‑1 (MQ=255)
                                gCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTtgct                                                           <  1:603851/90‑1 (MQ=255)
                                gCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTtgct                                                           <  2:5483/90‑1 (MQ=255)
                                   atttCGTTGACGCCGGCTTGACCAAGCTTGCGGCCCTGTGGTGACAGAAAAATCTCCTTTGCACCTTCCCCCGCCGCGGCTTTTGCTGCa                                                        <  2:990815/88‑1 (MQ=255)
                                      tCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGa                                                     >  1:820534/1‑90 (MQ=255)
                                             cGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGt                                              <  2:508060/90‑1 (MQ=255)
                                             cGCCCCTTTGACAAACCTTCCGCCCCTGTGGTGGCAGATAAACCCCTTTGGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGt                                              <  2:980039/90‑1 (MQ=255)
                                              gCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTc                                             >  1:35518/1‑90 (MQ=255)
                                              gCCCGCTTGACCAAGCTTGCGTCCCTGTGGTGACAGATAAACCCCCTTTGCGCTTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTc                                             <  2:970051/90‑1 (MQ=255)
                                                   tttGATCAAGCTTGCGTCCCTGTGGTGACAAAAAAACCCCCTTTGGGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                        <  1:1077260/89‑1 (MQ=255)
                                                   cTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                        <  1:124085/90‑1 (MQ=255)
                                                   cTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                        <  2:890031/90‑1 (MQ=255)
                                                   cTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                        <  2:801116/90‑1 (MQ=255)
                                                   cTTGAAAAAGTTTCCGTCCCTGTGGTGACAGATAAACCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                        <  1:379151/90‑1 (MQ=255)
                                                    ttaaTCAAGCTTGTGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCaa                                       <  2:525144/87‑1 (MQ=255)
                                                             cTTGCGTCCTTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGGTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                              <  1:368139/90‑1 (MQ=255)
                                                             cTTGCGTCCTTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                              <  1:249803/90‑1 (MQ=255)
                                                             cTTGCGTCCTTGTGGTGACAGAAAAATCCCCTTTGCCCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                              <  1:671316/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                              <  1:50345/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                              <  2:490181/90‑1 (MQ=255)
                                                             cTTGCGCCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                              <  1:1033734/90‑1 (MQ=255)
                                                             cTTCCGCCCCTGGGGTGACAGATAAATCCCCTTCGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                              <  1:580976/90‑1 (MQ=255)
                                                                     ccTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTa                      >  2:665549/1‑90 (MQ=255)
                                                                     ccTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTa                      >  2:678475/1‑90 (MQ=255)
                                                                     ccTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTa                      >  1:228986/1‑90 (MQ=255)
                                                                     ccTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTa                      >  1:443812/1‑90 (MQ=255)
                                                                       tgtgGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAAc                    <  2:1012660/90‑1 (MQ=255)
                                                                          ggtgACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATc                 >  1:312890/1‑90 (MQ=255)
                                                                             gACAGATAAACCCCCTTCGCCCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATcccc              <  1:683035/90‑1 (MQ=255)
                                                                               cAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCgg            >  1:825319/1‑90 (MQ=255)
                                                                                     aaTCCCTTTTGCGCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgcc      <  1:403564/90‑1 (MQ=255)
                                                                                       tCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgccgc    <  1:678475/90‑1 (MQ=255)
                                                                                         aCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgccgccg  >  1:619610/1‑90 (MQ=255)
                                                                                         |                                                                                         
GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCG  >  NZ_CP009273/2738359‑2738537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: