| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,127,543 | 0 | T | G | 58.3% | 3.4 / 11.1 | 12 | T15P (ACC→CCC) | yijF | DUF1287 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (7/0); total (8/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCTGCGATGGCAAGGTTGGTATTGGCCTGGATTTGTACCACGGTTGGTGGAACGGCGGGAGATTTTAAGGAGTGGCTGGTAAATGCCGTTAGCAGGCTGAGCAGCGCCAGTGAAGCTTTCATCTGGTTCGTCCGTGAAAATAAGAGTCACAGATTCGACCTTC > NZ_CP009273/4127463‑4127626 | gTCTGCGATGGCAAGGTTGGTATTGGCCTGGATTTGTACCACGGTTGGTGGAACGGCGGGGGATTTTTAGGAGGGGGTGGGAAAAGccct > 2:55308/1‑88 (MQ=255)gTCTGCGATGGCAAGGTTGGTATTGGCCTGGATTTGTACCACGGTTGGTGGAACGGCGGGAGGTTTTTAGGAGGGGGTGGGGAAAGCCgg > 2:182198/1‑89 (MQ=255) tGGCAAGGTTGGTATTGGCCTGGATTTGTACCACGGTTGGTGGAACGGCGGGAGATTTTAAGGAGTGGCTGGTAAATGCCGTTAGCAGGc < 1:76567/90‑1 (MQ=255) ggATTTGTACCACGGTTGGTGGAACGGCGGGAGATTTTAAGGAGTGGGTGGGAAAAGCCGGTTGCAGGGTGGGCAGCGGCAGGGAAGGtt > 2:417171/1‑90 (MQ=255) ggATTTGTACCACGGTTGGTGGAACGGCGGGAGATTTTAAGGAGTGGCTGGTAAATGCCGTTAGCAGGCTGAGCAGCGCCAGTGAAGCtt < 1:844368/90‑1 (MQ=255) ggATTTGTACCACGGTTGGTGGAACGGCGGGAGATTTTAAGGAGTGGCTGGTAAATGCCGTTAGCAGGCTGAGCAGCGCCAGTGAAGCtt < 2:606431/90‑1 (MQ=255) ggATTTGTACCACGGTTGGTGGAACGGCGGGAGATTTTAAGGAGGGGCTGGGAAAATCCGTTTGCAGGCTGAGCAGCGCCCGGGaaagtt > 2:245712/1‑86 (MQ=255) tGTACCACGGTTGGTGGAACGGCGGGAGATTTTAAGGAGTGGCTGGTAAATGCCGTTAGCAGGCTGAGCAGCGCCAGTGAAGCTTTCATc < 1:746743/90‑1 (MQ=255) gTACCACGGTTGGTGGAACGGCGGGAGATTTTAAGGAGTGGCTGGGAAAAGCCGTTTGCAGGGTGAGCAGCGCCCGGGAAGCTTTTATCt > 2:288609/1‑90 (MQ=255) cACGGTTGGTGGAACGGCGGGGGATTTTAAGGAGGGGCTGGGAAAAGCCGGTTGCAGGCTGGGGAGCGCCCGGGAAAGTTTTATTTgggt > 2:130206/1‑88 (MQ=255) gTGGCTGGGAAATGCCGTTAGCAGGCTGAGCAGCGCCAGTGAAGGTTTCATCTGGGTCGTCCGGGAAAATAAGAGTCACAGATTCGACCt > 1:714356/1‑90 (MQ=255) ggCTGGTAAATGCCGTTAGCAGGCTGAGCAGCGCCAGTGAAGCTTTCATCTGGGTCGGCCGTGAAAAAAAGAGTCACACAGTCGACCTTc > 1:842759/1‑90 (MQ=255) | GTCTGCGATGGCAAGGTTGGTATTGGCCTGGATTTGTACCACGGTTGGTGGAACGGCGGGAGATTTTAAGGAGTGGCTGGTAAATGCCGTTAGCAGGCTGAGCAGCGCCAGTGAAGCTTTCATCTGGTTCGTCCGTGAAAATAAGAGTCACAGATTCGACCTTC > NZ_CP009273/4127463‑4127626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |