| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,290,979 | 0 | T | G | 60.0% | 8.7 / 16.1 | 20 | T639T (ACA→ACC) | mdtO | multidrug efflux transporter permease subunit MdtO |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (12/0); total (13/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAAC > NZ_CP009273/4290891‑4291060 | gggCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGgtg < 1:1074488/90‑1 (MQ=255) gTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGctct > 2:966050/1‑90 (MQ=255) gTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAg > 2:64653/1‑90 (MQ=255) cTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCg < 2:1098896/90‑1 (MQ=255) cTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGGCTCTTCAAGCg > 1:851122/1‑90 (MQ=255) gCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTTAAGCGttt > 2:309258/1‑89 (MQ=255) gCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCTAGCGttt > 1:715515/1‑89 (MQ=255) ggCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGgtg < 1:1034241/68‑1 (MQ=255) ggCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGgtg > 2:1034241/1‑68 (MQ=255) aaaGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAAc < 1:309258/90‑1 (MQ=255) aGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTTAAGCGGTTTTTTAGGGGAAAgg > 2:429910/1‑90 (MQ=255) tGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTTTTTTAGGGGAACGGcgg > 2:938948/1‑88 (MQ=255) ttttAATAAGGTGGGCAGGATGGCCTGCGCGGCCGGTGTCCCTCCAAGAGTTTGTTGATGAGACAGGCGGACTGCCGTTGCCAGAGCggt < 1:158999/90‑2 (MQ=255) taataaGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGc < 1:834865/90‑1 (MQ=255) ggTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTTAAGCGGTTTTTTAGGAGAACGGCTGGGTGGGGGTGCCCGACCGGCAGCGTAttt > 2:823120/1‑90 (MQ=255) ggTGGGCAGGATGGCCTGAGAGGCGGGGGTCTCTTCAAGCGTTATTTGAGGGGAACGGCGGGGGGGGGGTGCCAGAGCGGCAGGGGAttt > 1:384494/1‑90 (MQ=255) tGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGGTATTTGAGGGGAACGGCTGGGGGGGGTTGCCAGACCGGCGGCGGAttttt > 2:868132/1‑88 (MQ=255) gggCAGGATGGCCTGAGAGGCGGGGGTCTCTTTAAGCGTTTTTTGGGGGGAAAGGGTGGGGGGGGGTGGCCGACCGGCAGGGGATTtttc > 1:3276/1‑90 (MQ=255) aTGGCCTGAGAGGCGGGGGTCTCTTCAAGCGGTTTTTTAGGAGAAAGGCTGAGGGCGGGTGCCCGACCCGCAGGGGATTTTTCCCggggg > 2:721124/1‑88 (MQ=255) gCCTGAGAGGCGGGGGGGTCTTCTAACGGGTTTTGAGGGGAAAGGGTGGGGGGGGGTGCCCGACCGGGAGCGTATTTTTCCCGGgcggcg > 1:601047/1‑90 (MQ=255) gagGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAAc < 1:938948/90‑1 (MQ=255) gagGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAAc < 1:542668/90‑1 (MQ=255) gagGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAAc < 1:505210/90‑1 (MQ=255) | GGGCTGTCCAGTCTGGCAAGCGGGCGAAAAGCTGGCAGACGTGTTGCTCCTGTTTTAATAAGGTGGGCAGGATGGCCTGAGAGGCGGGTGTCTCTTCAAGCGTTATTTGAGGAGAACGGCTGAGTGCGGTTGCCAGACCGGCAGCGTATTTCTCCAGGGCGTCGGCAAAC > NZ_CP009273/4290891‑4291060 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |