| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,380,341 | G→A | intergenic (+31/‑72) | yfbL → / → yfbM | M28 family metallopeptidase/YfbM family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,341 | 0 | G | A | 76.5% | 14.0 / 4.4 | 17 | intergenic (+31/‑72) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base G (0/4); new base A (13/0); total (13/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.20e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.20e-03 | |||||||||||
AAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGG > NZ_CP009273/2380257‑2380404 | aaaaTGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACa < 1:316220/90‑1 (MQ=255) ggCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGGCAGAAAAAAAGACAAGcga > 1:520787/1‑88 (MQ=255) cAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAGGAAAAGCAAattt > 2:105101/1‑90 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACAATTTTTTTTTCCTcc > 2:153912/1‑89 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGAAAAGCACATTTTTTTTTTCCTcc > 2:633909/1‑89 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 2:400653/90‑1 (MQ=255) cTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAGCACATTTTTTTTTTTCTCCTCttttttta > 1:370158/1‑88 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCtttttttaa > 2:191506/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAGCACATTTTTTTTTTTCTCCTCtttttttaa > 1:314225/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAGCACAATTTTTTTTTCCTCCTCtttttttaa > 1:33656/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGGAAAGGACATTTTTTTTTTCCTCCTCtttttttaa > 2:23617/1‑87 (MQ=255) tttGCTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCATCTGtttttaa > 2:159890/1‑87 (MQ=255) taCAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGaa < 2:136957/90‑1 (MQ=255) aGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCTTTTTTTAGATGCGGAAAATGa > 2:161596/1‑90 (MQ=255) aaaTAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCCCCTCTTTTTTTAAATGCGAAAAAAGaaaa > 2:321598/1‑89 (MQ=255) ataaAAATTTCTCGAGGCGGGTCCCGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTTTTTTTAAATGGGAAAAAAAAAAATCAAc > 2:935683/1‑90 (MQ=255) aaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagg < 1:1009811/90‑1 (MQ=255) | AAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGG > NZ_CP009273/2380257‑2380404 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |