| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,269,384 | 0 | T | G | 52.9% | 12.8 / 19.7 | 17 | G326G (GGT→GGG) | ghxP | guanine/hypoxanthine transporter GhxP |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (9/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAC‑GGC‑‑GGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCT > NZ_CP009273/4269300‑4269473 | actgaccactgacTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGAc‑ggg > 1:134879/1‑89 (MQ=255)actgaccactgacTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGAc‑ggg > 1:972850/1‑89 (MQ=255) tgaccactgacTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGAc‑ggc‑‑gg > 2:371211/1‑90 (MQ=255) ctgacTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGAC‑Ggg‑‑gggggggg > 1:292136/1‑88 (MQ=255) ctgacTCCATGAGCAGCG‑TTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGAC‑GGC‑‑Ggggggggg > 1:528163/1‑90 (MQ=255) acTCCATGAGCAGCGTTTTCTCTGGCC‑GGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGGC‑ggg‑‑gggggggggggg > 2:117559/1‑87 (MQ=255) agcGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGACGGGG‑‑GGGGGGGGGGAAAAACGGttt > 1:1005158/1‑90 (MQ=255) agcGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGAC‑GGCGGGGGGGGGGGAAAAACCGGtt > 1:753998/1‑90 (MQ=255) tggtggGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGGAC‑GGCG‑GGGGGGGGGGAAAACCGGTTTG‑CGGGTTTCAcccctg > 1:1082643/1‑86 (MQ=255) tgCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAC‑GGC‑‑GGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCt < 2:216390/90‑1 (MQ=255) ggCAGCGGTATACATCGAGTCTGCGGCGGGTAC‑GGC‑‑GGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTcctcct < 1:255071/90‑1 (MQ=255) cGGTATACATCGAGTCTGCGGCGGGTAC‑GGC‑‑GGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTc < 1:1149226/90‑1 (MQ=255) aGTCTGCGGCGGGTAC‑GGC‑‑GGCGGGGGGGAAAAACGGGTTTTCGGCTATCACCCCTGGGGGGCTGTTTCTCCCGATTTTTTTtctctctc > 1:408133/1‑90 (MQ=255) cTGCGGCGGGTAC‑GGC‑‑GGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGc < 2:1005158/90‑1 (MQ=255) cTGCGGCGGGTAC‑GGC‑‑GGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGc < 2:801352/90‑1 (MQ=255) cTGCGGCGGGTAC‑GGC‑‑GGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGc < 2:496579/90‑1 (MQ=255) cTGCGGCGGGTAC‑GGC‑‑GGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGc < 2:221837/90‑1 (MQ=255) tAC‑GGC‑‑GGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCt < 2:379363/90‑1 (MQ=255) tAC‑GGC‑‑GGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCt < 2:615176/90‑1 (MQ=255) tAC‑GGC‑‑GGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCt < 2:211476/90‑1 (MQ=255) tAC‑GGC‑‑GGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCt < 2:972850/90‑1 (MQ=255) | ACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAC‑GGC‑‑GGCGGGCGGTAAAACCGGTTTGACGGCTATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCT > NZ_CP009273/4269300‑4269473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |