| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,957 | 0 | A | G | 57.1% | 15.5 / 28.2 | 28 | L494P (CTC→CCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (3/9); new base G (16/0); total (19/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.19e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAA > NZ_CP009273/822873‑823045 | tCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGgggggggggg > 1:467566/1‑90 (MQ=255) cATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAAc < 1:577732/90‑1 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGTGGACGCCGGGAg > 2:944018/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAAGCCGGGAg > 1:1105524/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAAGCCGGGAg > 1:995971/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAg > 1:13543/1‑90 (MQ=255) ggtggtGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCAACGCCCGAAgc > 1:1110047/1‑89 (MQ=255) gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCCGAAGGCGGGtc > 2:814400/1‑90 (MQ=255) gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAAGCCGGGAGGCgggtt > 1:1224806/1‑87 (MQ=255) aCCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGCACGCCGGGAGTCGGtttg > 2:721950/1‑88 (MQ=255) aCCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCCGAAGTCGGGtcg > 2:69016/1‑90 (MQ=255) tCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCCGGAGTCGGGtcgtcgg > 2:162776/1‑89 (MQ=255) tttCTCTACCATGCTGTTGACGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGAAGGCGGGTTGTCGGGAAACAGAATggc > 2:809277/1‑88 (MQ=255) ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc < 1:431545/90‑1 (MQ=255) ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc < 1:956956/90‑1 (MQ=255) ttCTCTACCATGCTGTTGATGGGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc < 2:1092122/90‑1 (MQ=255) tctcTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCg < 1:78309/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGGTCGTCGGGAAAAAAAATGTCCGGGTCATGc > 1:1083073/1‑90 (MQ=255) cTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGGTCATGCATc > 1:444899/1‑90 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCCGGAGACGGTTCGTTGAAAAAAAAAAATTCCGGGTTATTGATCAg > 1:1307449/1‑90 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAAAAAAAAGTCCGGGTCCTGCATCAg > 1:773809/1‑90 (MQ=255) gATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGAAGTCGGGTCGTCGAGAAACAAAATGTCCGGGTTATGCATCAGCgg > 1:1218416/1‑89 (MQ=255) gCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGGCGGGTCGTCGAGAAACAGAAAGGCCGGGTCATGCATCAGCGAACAgg > 1:284908/1‑90 (MQ=255) gCAGCCAAAATTCACGGCGGGGGAGGGGGGGAACGCCGGAAGGCGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGCCTCAGCGAACAgg > 2:1124204/1‑90 (MQ=255) aGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGGCGGTTCGTCGAGAAAAAGAAAGTCCGGGTCATGCATCAGCGAACAgggc > 2:1057938/1‑88 (MQ=255) aTTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGgccagcgcca < 2:1048863/90‑1 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGAGAACAGGCCAGCGCCAGCCGc < 1:809277/90‑1 (MQ=255) gAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTaaa < 1:162776/90‑1 (MQ=255) gAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACACAATGTCCGGTTCATGCATCACCGAACAGGCCAGCGCCAGCCGCTGTTTaaa < 2:977870/90‑1 (MQ=255) | TCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAA > NZ_CP009273/822873‑823045 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |