| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,727 | 0 | T | G | 59.1% | 8.8 / 20.8 | 22 | G165G (GGT→GGG) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (1/8); new base G (13/0); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.81e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152646‑1152816 | gCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGTAAAtaat > 2:576661/1‑87 (MQ=255) cTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATACTg > 2:828948/1‑90 (MQ=255) cgcgAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATAATGGGTCTC‑GGGttt > 1:244505/1‑90 (MQ=255) cGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATtgt < 2:646781/90‑1 (MQ=255) gCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGGGAAATAATTGGTCTC‑GGGTTTTAAATCTCCTTTTTCCGCCTTGTGACCt > 2:215296/1‑90 (MQ=255) gTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACAACTCCTTTTCCGGCATTGTGACCTTc < 2:83296/90‑1 (MQ=255) ctacACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGt < 1:1126750/90‑1 (MQ=255) acacTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATACTGGGTTTC‑GGGTTTTTTATTTCCTTTTTCCGCCTTTTGGCCTTTCCTaa > 1:1072430/1‑90 (MQ=255) acacTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATAATGGATCTT‑GGGTTTTTTATTTCCTTTTCCCGCATTGTTGCCTTTCTTaa > 2:884254/1‑90 (MQ=255) acTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAAATACTTGGTTTCGGGTTTTTAATTTTCCTTTTCC‑GCGTTGTGGCCTTCCCTAAtt > 1:702521/1‑89 (MQ=255) cTGTTTTACAGAGGACAGAGAAACGGC‑GGGGAAATTACTGGGTCTC‑GGGTTTTATATTTCCTTTTTCCGCATTTTGGCCTTTCCTAATGc > 2:258563/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGC‑GGGGAAATTACTGGATCTC‑GGGTTTTACATCTCCTTTTTCGGCCTTGTGGCCTTTCCTAATGCgg > 1:112134/1‑90 (MQ=255) cAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAAc < 2:467269/90‑1 (MQ=255) cAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAAc < 2:376210/90‑1 (MQ=255) cAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAAc < 2:345953/90‑1 (MQ=255) ggACAGAGAAACGGC‑GGGGGAAATACTGGGTTTT‑GGGTTTTTTATATCCTTTTTCCGCATTGTGGGCTTCCCTTATGCGGGGGAACAgcg > 2:940182/1‑90 (MQ=255) ggACAGAGAAACGGC‑GGGGAAATTACTGGGTCTC‑GGGTTTTTCATCTCCTTTTTCGGCATTGTGGCCCTCCGTAATGCGGGGGAACTgcg > 1:158244/1‑90 (MQ=255) ggACAGAGAAACGGC‑GGGGAAATTACTGGATCTC‑GGGTTTTAAATCTCCTTTTTCGGCATTGTGGCCTTTCGTAATGCGGGGGAAcaggg > 1:36997/1‑86 (MQ=255) ggACAGAGAAACGGC‑GGGGAAAATACTTGGTCTC‑CGGTTTTATATCTCCCTTTTCCGCATTGTGGCCCTCCCTAATTCGGGGGAACCgcc > 2:174562/1‑89 (MQ=255) ggACAGAGAAACGGC‑GGGGAAAATACTGGGTCTC‑GGGTTTTAAATCTCCTTTTTCCGCATTGTGGCCTTCCCTAATTCGGGGGAACcgcg > 1:282026/1‑90 (MQ=255) cAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGa < 2:1218240/90‑1 (MQ=255) gggTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTa < 2:393370/90‑1 (MQ=255) tAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:1072430/90‑1 (MQ=255) tAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:940182/90‑1 (MQ=255) tAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:668561/90‑1 (MQ=255) | GCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGC‑GGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152646‑1152816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |